Detailed information of TA system TA140759

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 2119554..2120083
• Replicon: chromosome
• Accession: NZ_CP044345
• Organism: Mycobacterium tuberculosis strain L

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: F6W99_RS10375
• Protein ID: WP_003417760.1
• Coordinates: 2119826..2120083 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: F6W99_RS10370
• Protein ID: WP_003417757.1
• Coordinates: 2119554..2119829 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6W99_RS10335	2116127..2116921	-	795	WP_003417738.1	BBE domain-containing protein	-
F6W99_RS10340	2117003..2117374	-	372	WP_003417739.1	FAD-binding oxidoreductase	-
F6W99_RS21680	2117272..2117490	-	219	WP_157132559.1	hypothetical protein	-
F6W99_RS10350	2117517..2117780	-	264	WP_003906084.1	hypothetical protein	-
F6W99_RS10355	2117892..2118281	+	390	WP_003417745.1	DUF732 domain-containing protein	-
F6W99_RS10360	2118295..2118588	-	294	WP_003417749.1	DUF3017 domain-containing protein	-
F6W99_RS10365	2118585..2119430	-	846	WP_003417751.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
F6W99_RS10370	2119554..2119829	+	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
F6W99_RS10375	2119826..2120083	+	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
F6W99_RS10380	2120125..2121315	+	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
F6W99_RS10385	2121432..2121800	+	369	WP_003417765.1	FHA domain-containing protein	-
F6W99_RS10390	2121797..2122348	-	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
F6W99_RS10395	2122355..2122936	-	582	WP_003417769.1	ATP/GTP-binding protein	-
F6W99_RS10400	2122917..2123285	-	369	WP_003417772.1	DUF742 domain-containing protein	-
F6W99_RS10405	2123263..2123655	-	393	WP_003417776.1	roadblock/LC7 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T140759 WP_003417760.1 NZ_CP044345:2119826-2120083 [Mycobacterium tuberculosis]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T140759 NZ_CP059414:2943989-2944168 [Enterobacter hormaechei]
ATGGATAGCAGGAATGCAATAGCAATGATAGAAGCCGATGGGTGGTATCTAGTGAGAGTTAAAGGCAGTCATCACCAGTT
CAAACACCCAACGAAAAAGGGGCTGGTAACGGTAAAGCATCCACAGAAAGACATACCGTTACCAACACTGAAAAGCATCA
AAAAACAGGCGGGGCTCTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT140759 WP_003417757.1 NZ_CP044345:2119554-2119829 [Mycobacterium tuberculosis]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT140759 NZ_CP059414:2944190-2944600 [Enterobacter hormaechei]
ATGTTGTACCCTGTTGCTATTGATAAAGGCGATTCATCCTTCGGCGTTCGCGTACCTGATATTCCTGGCTGCTTCTCTGG
CGGTGATGATTATCAGGACGCGATCGAAAGCGTACGTGAAGCGATCGAGGCACACATCGAATTACTGGTTGAAGATGGCG
AGAGCGTTCCGGAAGCAACGACCGTCGAAAACTGGCTATCTGATCCGGAATACACCGGTGCGGTATGGGCCCTGGTTGAC
GTGGATATAACTCGCCTGATGGGAAAAGCGGAAAAAATCAATGTGACGCTTCCCTCATTGCTGATCCGCCGTATCGATCA
GTTTGTGGCCGCGCATCCAGAATACGGCAGTCGGTCTGGCTTCCTGTCTCGTGTCGCGGCAGATAAAGTAATCGGACGAG
AAAAACGATAA

Structures

Toxin

• PDB: 3OEI

Antitoxin

• PDB: 3CTO
• PDB: 3OEI
• PDB: 3D55
• AlphaFold DB: G0TI94