Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 1477696..1478300 Replicon chromosome
Accession NZ_CP044345
Organism Mycobacterium tuberculosis strain L

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag F6W99_RS07395 Protein ID WP_003414492.1
Coordinates 1477696..1478088 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag F6W99_RS07400 Protein ID WP_003414495.1
Coordinates 1478085..1478300 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F6W99_RS07365 1472846..1473568 - 723 WP_003912014.1 CRISPR-associated endoribonuclease Cas6 -
F6W99_RS07370 1473968..1474513 - 546 WP_003913758.1 DUF1802 family protein -
F6W99_RS07375 1474785..1475669 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
F6W99_RS07380 1475672..1476559 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
F6W99_RS07385 1476864..1477409 - 546 WP_003913758.1 DUF1802 family protein -
F6W99_RS07390 1477406..1477675 - 270 WP_003912856.1 DUF2277 family protein -
F6W99_RS07395 1477696..1478088 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
F6W99_RS07400 1478085..1478300 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
F6W99_RS07405 1478347..1479096 + 750 WP_003414497.1 enoyl-CoA hydratase -
F6W99_RS07410 1479175..1480257 - 1083 WP_003414499.1 ABC transporter ATP-binding protein -
F6W99_RS07415 1480250..1481560 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
F6W99_RS07420 1481563..1482390 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
F6W99_RS07425 1482387..1483298 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T140754 WP_003414492.1 NZ_CP044345:c1478088-1477696 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T140754 NZ_CP059414:54222-54593 [Enterobacter hormaechei]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGGGCGCTCGCAGGCGTGGTCATGAAG
CGAAAACGGCAGCCGTGATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTCACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT140754 WP_003414495.1 NZ_CP044345:c1478300-1478085 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT140754 NZ_CP059414:53977-54219 [Enterobacter hormaechei]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGGGAAGCGATAGAACTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References