Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1450012..1450582 Replicon chromosome
Accession NZ_CP044345
Organism Mycobacterium tuberculosis strain L

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag F6W99_RS07235 Protein ID WP_003414166.1
Coordinates 1450012..1450368 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag F6W99_RS07240 Protein ID WP_003901465.1
Coordinates 1450352..1450582 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F6W99_RS07215 1445464..1447152 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
F6W99_RS07220 1447156..1447482 - 327 WP_003414157.1 hypothetical protein -
F6W99_RS07225 1447613..1448242 + 630 WP_003414158.1 DUF3558 family protein -
F6W99_RS07230 1448261..1449910 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
F6W99_RS07235 1450012..1450368 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
F6W99_RS07240 1450352..1450582 - 231 WP_003901465.1 antitoxin MazE Antitoxin
F6W99_RS07245 1450625..1451668 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
F6W99_RS07250 1451859..1452134 + 276 WP_003911993.1 DUF1778 domain-containing protein -
F6W99_RS07255 1452163..1452354 - 192 WP_003918504.1 hypothetical protein -
F6W99_RS07265 1452565..1452969 + 405 WP_009938577.1 hypothetical protein -
F6W99_RS07270 1452966..1453157 + 192 WP_003414184.1 hypothetical protein -
F6W99_RS07275 1453356..1454510 + 1155 WP_003899488.1 DDE-type integrase/transposase/recombinase -
F6W99_RS07280 1454744..1455001 + 258 WP_003899489.1 hypothetical protein -
F6W99_RS07285 1455106..1455417 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T140752 WP_003414166.1 NZ_CP044345:c1450368-1450012 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T140752 NZ_CP059413:93667-93954 [Enterobacter hormaechei]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT140752 WP_003901465.1 NZ_CP044345:c1450582-1450352 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT140752 NZ_CP059413:93428-93667 [Enterobacter hormaechei]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References