Detailed information of TA system TA140750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1410015..1410694
• Replicon: chromosome
• Accession: NZ_CP044345
• Organism: Mycobacterium tuberculosis strain L

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: F6W99_RS07010
• Protein ID: WP_003414059.1
• Coordinates: 1410015..1410431 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: F6W99_RS07015
• Protein ID: WP_003414061.1
• Coordinates: 1410428..1410694 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6W99_RS06990	1406067..1406969	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
F6W99_RS06995	1407038..1407790	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
F6W99_RS07000	1408034..1408309	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
F6W99_RS07005	1408306..1409928	-	1623	WP_003906897.1	type I restriction-modification system subunit M	-
F6W99_RS07010	1410015..1410431	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
F6W99_RS07015	1410428..1410694	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
F6W99_RS07020	1410720..1411115	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
F6W99_RS07025	1411112..1411381	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
F6W99_RS07030	1411391..1412485	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
F6W99_RS07035	1412482..1412901	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
F6W99_RS07040	1412975..1413454	-	480	WP_003414073.1	dihydrofolate reductase	-
F6W99_RS07045	1413525..1414325	-	801	WP_003911953.1	thymidylate synthase	-
F6W99_RS07050	1414481..1415218	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T140750 WP_003414059.1 NZ_CP044345:c1410431-1410015 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T140750 NZ_CP059405:2366538-2366888 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT140750 WP_003414061.1 NZ_CP044345:c1410694-1410428 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT140750 NZ_CP059405:2366251-2366532 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64