Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1220394..1221031 Replicon chromosome
Accession NZ_CP044345
Organism Mycobacterium tuberculosis strain L

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag F6W99_RS05950 Protein ID WP_003413180.1
Coordinates 1220394..1220789 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag F6W99_RS05955 Protein ID WP_003413183.1
Coordinates 1220786..1221031 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F6W99_RS05905 1215797..1217008 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
F6W99_RS05915 1217135..1217794 + 660 WP_003900846.1 LppA family lipoprotein -
F6W99_RS05920 1217791..1218453 + 663 WP_003900848.1 hypothetical protein -
F6W99_RS05925 1218450..1218728 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
F6W99_RS05930 1218821..1219234 + 414 WP_003912787.1 PIN domain nuclease -
F6W99_RS05935 1219273..1219530 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
F6W99_RS05940 1219527..1219904 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
F6W99_RS05945 1219920..1220294 - 375 WP_003413177.1 hypothetical protein -
F6W99_RS05950 1220394..1220789 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
F6W99_RS05955 1220786..1221031 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
F6W99_RS05965 1221442..1221861 - 420 WP_003413190.1 A24 family peptidase -
F6W99_RS05970 1221873..1222682 - 810 WP_003413193.1 shikimate dehydrogenase -
F6W99_RS05975 1222679..1223932 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
F6W99_RS05980 1223925..1224437 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T140748 WP_003413180.1 NZ_CP044345:c1220789-1220394 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T140748 NZ_CP059400:864707-864988 [Legionella sp. PC1000]
ATGCTTGAATTAGAATTAGCCACTCAATTTAAGCGCGATTTAAAAAAGATAGTGAAGCAGGGGAAGGATAGAAAGCTAAT
CGATTCGATTGTGGGGCAATTGCAAAGGGAAGAACCATTGCACCCAAAGCATCAAGATCATAACCTTTCTGGAAATTGGC
ATGGCTATCGTGAATGTCACATCACCCCTGATTGGCTATTAATTTATAAAGTCATTAAGGATGAGTGTATGCAGTTGCTA
AGATTATCGAGAACTGGCTCTCATTCTGATTTGTTTAAATAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT140748 WP_003413183.1 NZ_CP044345:c1221031-1220786 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT140748 NZ_CP059400:864454-864714 [Legionella sp. PC1000]
ATGAGTAAGGCAGCGACAATTAATGCTCGTATTGAGCCTGCTCTTAAAATGCAGGCGGAAGCGATATTGCATAAAGTAGG
TTTATCTACAGCCGAGGCAATTCGACTGTTCTATAGTCAAGTTTGTCTTCAAAATGGTCTCCCGTTTGAGGTAAAAATAC
CGAACAAGAAGACACTTGATGCAATTAAAGAGCTTGAATCAGGTAAAGGAGAGCGATTTAAAACCATGAAGGATGTTTGG
GATTCTATAGATAATGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References