Detailed information of TA system TA140745

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1204932..1205572
• Replicon: chromosome
• Accession: NZ_CP044345
• Organism: Mycobacterium tuberculosis strain L

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: F6W99_RS05840
• Protein ID: WP_003412970.1
• Coordinates: 1204932..1205351 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: F6W99_RS05845
• Protein ID: WP_003412975.1
• Coordinates: 1205348..1205572 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6W99_RS05810	1200517..1201239	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
F6W99_RS05820	1201756..1201983	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
F6W99_RS05825	1201980..1202381	+	402	WP_003412963.1	PIN domain-containing protein	-
F6W99_RS05830	1202416..1203336	-	921	WP_003412965.1	restriction endonuclease	-
F6W99_RS21640	1203677..1203922	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
F6W99_RS05835	1203981..1204931	+	951	WP_003911916.1	Lsr2 family protein	-
F6W99_RS05840	1204932..1205351	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
F6W99_RS05845	1205348..1205572	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
F6W99_RS05850	1205603..1208446	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
F6W99_RS05855	1208518..1208919	-	402	WP_003412981.1	hypothetical protein	-
F6W99_RS05860	1208919..1209389	-	471	WP_003899364.1	transcription antitermination factor NusB	-
F6W99_RS05865	1209392..1209955	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T140745 WP_003412970.1 NZ_CP044345:c1205351-1204932 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T140745 NZ_CP059399:1978187-1978540 [Nocardia sp. WCH-YHL-001]
TTGAAATTCGTCGTAGATGCCCAGCTGCCACCAGCGCTGGCCCGTGCGCTGGCTGCCGAAGGACATGAGGCTGTTCACGT
CTGCGATCTTGGAATGACCTCTGCGTCTGATCGCGAGGTATGGAACGAGGCGCTCGAGCGCGAGGCAGTCATCGTGACGA
AGGATGAAGACTTCGTCACGATCGGTCGTGCCCAGCCGAATGAGCCTGTGCCCGCGATTGTTTGGATTCGTATCGGAAAT
TGCTCGCGGAAGGCGTTGCTCGAATCGCTGTTGCCATTGCTGCCGACAGTGGTGGAGCTACTGGAGGCTGGGGAGCGCAT
CGTCGAGATACGGTCTCCGCAAACTCATTCCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT140745 WP_003412975.1 NZ_CP044345:c1205572-1205348 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT140745 NZ_CP059399:1977966-1978190 [Nocardia sp. WCH-YHL-001]
GTGGCGGTGTCCAGGATTACGTTCGACCCGAATCAGCTCGGGGGCCGGCCGTGTATCCGCGGTATGCGGATCCGGGTGAA
GGACGTTCTCGAACTCCTCGCCGCTGGTGAAACCGAGGACTCGATCCTCGAGGACTACCCGTATCTGGAGCGGGAGGATA
TTCGCGCCTGCCTGGCGTTTGCCGCGGCGGAAGCAGATCACCCGATCTTGCGGCCCGCATCTTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09