Detailed information of TA system TA140739

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 605267..605908
• Replicon: chromosome
• Accession: NZ_CP044345
• Organism: Mycobacterium tuberculosis strain L

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: F6W99_RS02910
• Protein ID: WP_003410075.1
• Coordinates: 605510..605908 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WLM7
• Locus tag: F6W99_RS02905
• Protein ID: WP_003899127.1
• Coordinates: 605267..605509 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6W99_RS02895	603321..603665	-	345	WP_003410065.1	ferredoxin family protein	-
F6W99_RS02900	603854..605179	-	1326	WP_003410070.1	DUF4143 domain-containing protein	-
F6W99_RS02905	605267..605509	+	243	WP_003899127.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
F6W99_RS02910	605510..605908	+	399	WP_003410075.1	PIN domain nuclease	Toxin
F6W99_RS02915	606091..606522	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
F6W99_RS02920	606563..607057	+	495	WP_003410080.1	DUF1398 domain-containing protein	-
F6W99_RS02935	607494..607673	-	180	Protein_562	hypothetical protein	-
F6W99_RS21595	607698..608924	+	1227	Protein_563	IS110 family transposase	-
F6W99_RS02950	609052..610308	-	1257	WP_003410095.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (7-48)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T140739 WP_003410075.1 NZ_CP044345:605510-605908 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T140739 NZ_CP059391:c4714526-4714242 [Klebsiella africana]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCTTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATAGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGCCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8851.73 Da
• Isoelectric Point: 4.1621

>AT140739 WP_003899127.1 NZ_CP044345:605267-605509 [Mycobacterium tuberculosis]
VYSGVVSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 243 bp

>AT140739 NZ_CP059391:c4714758-4714516 [Klebsiella africana]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCAGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGCGATG
GCGAAGCGGCGTTGTTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGACTCTGGATGACCTA
TGA

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A045JPF9