Detailed information of TA system TA140738

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 581540..582126
• Replicon: chromosome
• Accession: NZ_CP044345
• Organism: Mycobacterium tuberculosis strain L

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: F6W99_RS02810
• Protein ID: WP_003912702.1
• Coordinates: 581540..581884 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL58
• Locus tag: F6W99_RS02815
• Protein ID: WP_003410014.1
• Coordinates: 581878..582126 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6W99_RS02770	577246..577845	+	600	WP_003409989.1	L-lysine exporter	-
F6W99_RS02775	578261..578689	+	429	WP_003409992.1	cellulose-binding protein	-
F6W99_RS02780	578915..579454	+	540	WP_003900446.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37)	-
F6W99_RS02790	579974..580534	-	561	WP_003410001.1	RES family NAD+ phosphorylase	-
F6W99_RS02795	580531..580872	-	342	WP_003410003.1	DUF2384 domain-containing protein	-
F6W99_RS02800	580958..581215	+	258	WP_003410006.1	hypothetical protein	-
F6W99_RS02805	581116..581451	-	336	WP_003410009.1	dehydrogenase	-
F6W99_RS02810	581540..581884	-	345	WP_003912702.1	type II toxin-antitoxin system toxin endoribonuclease MazF6	Toxin
F6W99_RS02815	581878..582126	-	249	WP_003410014.1	ribbon-helix-helix protein, CopG family	Antitoxin
F6W99_RS02820	582226..584541	-	2316	WP_003899120.1	cation transporter ATPase CptG	-
F6W99_RS02825	584538..584810	-	273	WP_003410017.1	DUF1490 family protein	-
F6W99_RS02830	584863..585219	-	357	WP_003410018.1	Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR	-
F6W99_RS02835	585376..586143	+	768	WP_003410019.1	hemerythrin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-38)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12260.15 Da
• Isoelectric Point: 9.8887

>T140738 WP_003912702.1 NZ_CP044345:c581884-581540 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPSNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Nucleotide

• Length: 345 bp

>T140738 NZ_CP059391:c4629346-4629026 [Klebsiella africana]
ATGCAAATCTCAACTGTACCGGCCACGATGCCGGTTTCGTCACGCCTGTCGCCCGTGCAGGTCTGGCAGCAATTGTTAAC
GTATCTGCTGGAGCACCATTACGGCCTGACGCTCAACGACACACCATTCCATGATGATGCGGCCATCAAGGAACATATCG
AGGCGGGTATCACGCTTGCTGATGCGGTGAATTTCCTGGTGGAACGTTATGAACTGGTACGCACTGATCGCAAGGGGTTT
ACGTGGCAGGAGCAGACACCATTTGTAACCGCTACAGATATTCTCAGAGCCAGAAGAGCTACCGGGCTGATGAATAAGTA
A

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9290.34 Da
• Isoelectric Point: 4.3757

>AT140738 WP_003410014.1 NZ_CP044345:c582126-581878 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Nucleotide

• Length: 249 bp

>AT140738 NZ_CP059391:c4629749-4629414 [Klebsiella africana]
ATGTATAAAGACGCTACACCAACCTGGGGGCTTAGACGAGATATCACTCCGTGTTTCGGAGCCCGACTGGTGCAGGAAGG
TCATCGCCTGCATTTTCTGGCTAACCGGGCAGGATTCACCGGCTCATTCAGTGAGGTGCAGACCTTACAACTGAATGAAG
CCTTCCCGCATTTTGTCGCATATCTGGAGCTGATGCTGCTCTCCTGCGAGCTGAATCCCCGGTACGCGCACTGTGTCACA
CTGTACCGTAACGGGTTGACTTGTGAAGCAGATACGCTGGGCAGTCATGGGCACGTGTATATGGCAATTTATCCCTCAAA
TCAGGTTAAAGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4FAW9