Detailed information of TA system TA140737

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mbcAT/RES-TIGR02293
• Location: 579974..580872
• Replicon: chromosome
• Accession: NZ_CP044345
• Organism: Mycobacterium tuberculosis strain L

Toxin (Protein)

• Gene name: mbcT
• Uniprot ID: P64908
• Locus tag: F6W99_RS02790
• Protein ID: WP_003410001.1
• Coordinates: 579974..580534 (-)
• Length: 187 a.a.

Antitoxin (Protein)

• Gene name: mbcA
• Uniprot ID: P64910
• Locus tag: F6W99_RS02795
• Protein ID: WP_003410003.1
• Coordinates: 580531..580872 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6W99_RS02755	575143..575796	-	654	WP_003409976.1	cutinase Cfp21	-
F6W99_RS02760	575911..576141	+	231	WP_003409980.1	DUF167 domain-containing protein	-
F6W99_RS02765	576226..577137	-	912	WP_003409986.1	ArgP/LysG family DNA-binding transcriptional regulator	-
F6W99_RS02770	577246..577845	+	600	WP_003409989.1	L-lysine exporter	-
F6W99_RS02775	578261..578689	+	429	WP_003409992.1	cellulose-binding protein	-
F6W99_RS02780	578915..579454	+	540	WP_003900446.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37)	-
F6W99_RS02790	579974..580534	-	561	WP_003410001.1	RES family NAD+ phosphorylase	Toxin
F6W99_RS02795	580531..580872	-	342	WP_003410003.1	DUF2384 domain-containing protein	Antitoxin
F6W99_RS02800	580958..581215	+	258	WP_003410006.1	hypothetical protein	-
F6W99_RS02805	581116..581451	-	336	WP_003410009.1	dehydrogenase	-
F6W99_RS02810	581540..581884	-	345	WP_003912702.1	type II toxin-antitoxin system toxin endoribonuclease MazF6	-
F6W99_RS02815	581878..582126	-	249	WP_003410014.1	ribbon-helix-helix protein, CopG family	-
F6W99_RS02820	582226..584541	-	2316	WP_003899120.1	cation transporter ATPase CptG	-
F6W99_RS02825	584538..584810	-	273	WP_003410017.1	DUF1490 family protein	-
F6W99_RS02830	584863..585219	-	357	WP_003410018.1	Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (25-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (69-110)

Sequences

Toxin

Protein

• Length: 187 a.a.
• Molecular weight: 20248.70 Da
• Isoelectric Point: 4.5091

>T140737 WP_003410001.1 NZ_CP044345:c580534-579974 [Mycobacterium tuberculosis]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT

Nucleotide

• Length: 561 bp

>T140737 NZ_CP059391:3989271-3989489 [Klebsiella africana]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGCCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCAGCCGATCATCGCCTGGCTGAAC
TGACTATGAATAAGCTCTACGATAAGATCCCGACCTCGGTCTGGAAGTTTATCCGCTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12488.33 Da
• Isoelectric Point: 4.8359

>AT140737 WP_003410003.1 NZ_CP044345:c580872-580531 [Mycobacterium tuberculosis]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Nucleotide

• Length: 342 bp

>AT140737 NZ_CP059391:3988870-3989244 [Klebsiella africana]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGGTGGGTTAACGACCCGACATCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACGTTCGCGCTTAATTATAAAATTAAGTATGCGGAGGATAATAAACTGATCGATCAAGTTGACGAATATCTG
GACGATACTTTCACATTGTTCAGTAACTACGGCATTAACTCCTCGGATTTGCAGAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAATCCGGCTAGTCTTTCGTGTTAG

Structures

Toxin

• PDB: 6FKG

Antitoxin

• AlphaFold DB: A0A7U4FAE4