Detailed information of TA system TA140733

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 541872..542575
• Replicon: chromosome
• Accession: NZ_CP044345
• Organism: Mycobacterium tuberculosis strain L

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: F6W99_RS02520
• Protein ID: WP_003409778.1
• Coordinates: 541872..542201 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: F6W99_RS02525
• Protein ID: WP_003409780.1
• Coordinates: 542198..542575 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6W99_RS02500	538255..539325	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
F6W99_RS02505	539322..539837	+	516	WP_003409718.1	flavin reductase family protein	-
F6W99_RS02510	539834..540895	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
F6W99_RS02515	540892..541662	+	771	WP_003409775.1	SDR family oxidoreductase	-
F6W99_RS02520	541872..542201	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
F6W99_RS02525	542198..542575	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
F6W99_RS02530	542572..543162	-	591	WP_003409784.1	SEC-C domain-containing protein	-
F6W99_RS02535	543217..544581	+	1365	WP_003903691.1	HNH endonuclease	-
F6W99_RS02540	544736..545188	-	453	WP_003899095.1	lipoprotein	-
F6W99_RS02545	545252..545653	+	402	WP_003409869.1	hypothetical protein	-
F6W99_RS02550	545646..545828	-	183	WP_003409870.1	hypothetical protein	-
F6W99_RS02555	545942..546292	-	351	WP_003899096.1	hypothetical protein	-
F6W99_RS02560	546303..547205	-	903	WP_003899097.1	hypothetical protein	-
F6W99_RS02565	547226..547417	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T140733 WP_003409778.1 NZ_CP044345:c542201-541872 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T140733 NZ_CP059391:808310-808720 [Klebsiella africana]
GTGGTCCTGTGGCAATCTGATCTGCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTCCACGGCGTGGTGGCCGC
GCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTACACTCCGATCTGGCTGCTGCTGTTGTCGCTGGTGGTGTTTGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCTCGTCGCGGGGAGATAAAACTGCTCACCGACTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTTGGGACCCCGTGGGTCATCAACAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT140733 WP_003409780.1 NZ_CP044345:c542575-542198 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT140733 NZ_CP059391:808063-808329 [Klebsiella africana]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGATGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TATTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTACAGCGGATGGTAACCTTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8