Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 304358..304971 Replicon chromosome
Accession NZ_CP044345
Organism Mycobacterium tuberculosis strain L

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag F6W99_RS01370 Protein ID WP_003408465.1
Coordinates 304358..304747 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag F6W99_RS01375 Protein ID WP_003408469.1
Coordinates 304744..304971 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F6W99_RS01340 299988..300902 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
F6W99_RS01345 300905..301735 + 831 WP_003408448.1 cyclase family protein -
F6W99_RS01350 301735..302085 + 351 WP_003898986.1 cupin domain-containing protein -
F6W99_RS01355 302138..302956 + 819 WP_003408456.1 3-keto-5-aminohexanoate cleavage protein -
F6W99_RS01360 302970..303748 + 779 Protein_265 IclR family transcriptional regulator -
F6W99_RS01370 304358..304747 - 390 WP_003408465.1 PIN domain-containing protein Toxin
F6W99_RS01375 304744..304971 - 228 WP_003408469.1 antitoxin Antitoxin
F6W99_RS01380 305189..306673 + 1485 WP_003408473.1 carboxylase -
F6W99_RS01385 306670..307917 + 1248 WP_003408476.1 serine hydrolase -
F6W99_RS01390 307960..308379 - 420 WP_003408483.1 hypothetical protein -
F6W99_RS01395 308369..309079 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T140731 WP_003408465.1 NZ_CP044345:c304747-304358 [Mycobacterium tuberculosis]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T140731 NZ_CP059391:292319-292594 [Klebsiella africana]
ATGGAGCAGCAGGTTTTATCTTTACGTAATAAACAGCGCCAGACGCTGGAGCAACTGTTTAAAATCCCTGTGCCACAGGG
CATTAAATGGGCCGACATTGAATCGCTAATTAAAGCGTTAGGCGGGGAGATTAAAGAAGGACGCGGCTCGCGCTGTAAAT
TTTTGTTGAACCACAGCATTGCCAGCTTTCACAGGCCTCATCCTTCGCCGGATACCGACAAAGGCGCCGTGGAGAGCGTG
CGCGATTGGTTAATCACTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT140731 WP_003408469.1 NZ_CP044345:c304971-304744 [Mycobacterium tuberculosis]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT140731 NZ_CP059391:292591-292950 [Klebsiella africana]
ATGATCAAACCTAAAACGCCAAACTCAATGGAGATTGCCGGCCAGCCTGCCGTTATCAATTACGTGCCTGAGCTCAATGC
ATTCCGCGGTAAGTTCCTTGGATTATCAGGATATTGCGATTTTGTTTCCGATAGTATTCAAGGGTTACAACAGGAGGGCG
AGATATCATTACAAGAGTATCTCGCAGATTGTCATGAAGCGGGGATTGAGCCTTATGCTCACCCGGAAAAGATGAAAACC
TTCACGCTGCGCTATCCTGAGTCATTTGGCGAGCGTCTTAGCTCCGCCGCTGCGGAGGAGCAGGTTTCCGTAAATACCTG
GATCCTTGAGACGCTCAGTGAACGTCTCAAACAGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References