Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 50060..50676 Replicon chromosome
Accession NZ_CP044345
Organism Mycobacterium tuberculosis strain L

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag F6W99_RS00240 Protein ID WP_003407593.1
Coordinates 50359..50676 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag F6W99_RS00235 Protein ID WP_003900349.1
Coordinates 50060..50362 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F6W99_RS00225 45946..47793 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
F6W99_RS00230 47794..50046 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
F6W99_RS00235 50060..50362 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
F6W99_RS00240 50359..50676 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
F6W99_RS00245 50673..51677 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
F6W99_RS00250 51730..53019 + 1290 WP_003407599.1 serine hydrolase -
F6W99_RS00255 53092..53727 - 636 WP_071854212.1 class I SAM-dependent methyltransferase -
F6W99_RS00260 53923..54135 - 213 WP_003898900.1 dodecin family protein -
F6W99_RS21560 54145..54369 - 225 WP_160522526.1 NUDIX domain-containing protein -
F6W99_RS00270 54639..55667 + 1029 WP_003407612.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T140729 WP_003407593.1 NZ_CP044345:50359-50676 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T140729 NZ_CP059389:c3405-3085 [Acinetobacter baumannii]
ATGTTATTCATTGAAACCAGTATTTTTACTAAGCAAATCAAAGAGCTTGTGAGTGATGAAGAGTATCGTCAGCTCCAGCA
AGATCTCTTGGTACAGCCTGATAAGGGCGACTTAATCAAGAATGGTGGTGGAATTCGTAAAGTACGTTGTGCTCAGGGCA
ATAAAGGCAAAAGTGGCGGTATCCGTGTGATCTATTATTGGGTTACAGAAGATGATCAGATCTTTTTCCTGGTGGCTTAT
CCAAAGTCTGTGAAAGATAATCTGACAGACAAAGAAACCTCGATTCTACGTCAATTAGTAAAGGAGCAATTTCATGGATA
A

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT140729 WP_003900349.1 NZ_CP044345:50060-50362 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT140729 NZ_CP059389:c3092-2820 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACCTGGTTGCTTCAATCAAAGAAGCTGGTGCCATCAAGCGTAAGGAGATAAAGGCAAG
TCGAGTCACAGAGCTTGAATTACCGGATATTAAAGAAGTACGTGAGAAAACAGGCTTAACCCAGGCTGAATTTGCTGCAC
GTTTGCATATCAGCGCGAGAACCCTGCAAAACTGGGAACAAGGCCGTCGCTATCCGACTGGTCCTGCAGCGACTTTAATT
CGTATTTTAGATGCTCATCCAACTTTAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References