Detailed information of TA system TA140728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 957577..958136
• Replicon: chromosome
• Accession: NZ_CP044342
• Organism: Brucella melitensis strain RM57

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A7U8PD14
• Locus tag: F7D16_RS05030
• Protein ID: WP_002966807.1
• Coordinates: 957891..958136 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: C0RIV1
• Locus tag: F7D16_RS05025
• Protein ID: WP_004683740.1
• Coordinates: 957577..957891 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D16_RS04975	953282..953485	-	204	WP_004683737.1	hypothetical protein	-
F7D16_RS04980	953488..953703	-	216	WP_005968947.1	hypothetical protein	-
F7D16_RS04985	953700..953903	-	204	WP_002964088.1	hypothetical protein	-
F7D16_RS04990	953953..954657	+	705	WP_005968944.1	hypothetical protein	-
F7D16_RS04995	954654..954884	+	231	WP_002964091.1	hypothetical protein	-
F7D16_RS05000	954881..955156	+	276	WP_002964092.1	hypothetical protein	-
F7D16_RS05005	955179..955655	+	477	WP_002966806.1	hypothetical protein	-
F7D16_RS05010	956001..956711	+	711	WP_004683739.1	porin family protein	-
F7D16_RS05015	957059..957229	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
F7D16_RS05025	957577..957891	-	315	WP_004683740.1	BrnA antitoxin family protein	Antitoxin
F7D16_RS05030	957891..958136	-	246	WP_002966807.1	BrnT family toxin	Toxin
F7D16_RS05035	958269..958907	+	639	Protein_923	tRNA-guanine transglycosylase	-
F7D16_RS05040	958977..959735	+	759	Protein_924	IS5 family transposase	-
F7D16_RS05045	959736..960428	+	693	WP_006135935.1	hypothetical protein	-
F7D16_RS05050	960840..962291	+	1452	WP_006135932.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	952270..965607	13337
	-	flank	IS/Tn	-	-	958977..959345	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9162.82 Da
• Isoelectric Point: 10.3158

>T140728 WP_002966807.1 NZ_CP044342:c958136-957891 [Brucella melitensis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Nucleotide

• Length: 246 bp

>T140728 NZ_CP059386:c3354287-3353931 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAACGAATGGCTTGCACAGCAAGATCAATCTACACAAGAGAAAGTACTCGC
AGCGTTGGTCGTTCTACAGCAACAGGGACCAAGTTTAGGTCGTCCTTTAGTAGATACCGTCTATGACTCCAAATTTACCA
ATATGAAAGAGCTACGTGTTCAGCATCGAGGTAAACCACTGAGAGCCTTTTTCGCGTTTGATCCTTTACGACAAGCTATT
GTGCTGTGTATCGGTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGAGATGTTAGATATTGCCGATCAGCAATACGAAAT
TCATCTTTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11653.48 Da
• Isoelectric Point: 10.5824

>AT140728 WP_004683740.1 NZ_CP044342:c957891-957577 [Brucella melitensis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT140728 NZ_CP059386:c3353938-3353636 [Acinetobacter baumannii]
ATGGCTAAGACCCTACAAGAATTATTGGCTAATCGCTCTCCAGAGAGTCAAGCTCGTATTCAACAAATGGCGGATGAATT
ATTGTTGGAAACTCAGCTTCACCTTATTCGTGAAGAGTTAGAGATTTCTCAAAAAGAACTGGCTGCAACCCTTGGAATCA
AACAGCCTTCATTGTCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTCGAAGCAATG
GGCGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGATTCAATGTCTAA

Structures

Toxin

• AlphaFold DB: A0A7U8PD14

Antitoxin

• AlphaFold DB: C0RIV1