Detailed information of TA system TA140727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 961317..961876
• Replicon: chromosome
• Accession: NZ_CP044340
• Organism: Brucella melitensis strain M1981

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A7U8PD14
• Locus tag: F6Y32_RS05040
• Protein ID: WP_002966807.1
• Coordinates: 961631..961876 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: C0RIV1
• Locus tag: F6Y32_RS05035
• Protein ID: WP_004683740.1
• Coordinates: 961317..961631 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6Y32_RS04985	957022..957225	-	204	WP_004683737.1	hypothetical protein	-
F6Y32_RS04990	957228..957443	-	216	WP_005968947.1	hypothetical protein	-
F6Y32_RS04995	957440..957643	-	204	WP_002964088.1	hypothetical protein	-
F6Y32_RS05000	957693..958397	+	705	WP_005968944.1	hypothetical protein	-
F6Y32_RS05005	958394..958624	+	231	WP_002964091.1	hypothetical protein	-
F6Y32_RS05010	958621..958896	+	276	WP_002964092.1	hypothetical protein	-
F6Y32_RS05015	958919..959395	+	477	WP_002966806.1	hypothetical protein	-
F6Y32_RS05020	959741..960451	+	711	WP_004683739.1	porin family protein	-
F6Y32_RS05025	960799..960969	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
F6Y32_RS05035	961317..961631	-	315	WP_004683740.1	BrnA antitoxin family protein	Antitoxin
F6Y32_RS05040	961631..961876	-	246	WP_002966807.1	BrnT family toxin	Toxin
F6Y32_RS05045	962009..962647	+	639	Protein_923	tRNA-guanine transglycosylase	-
F6Y32_RS05050	962717..963475	+	759	Protein_924	IS5 family transposase	-
F6Y32_RS05055	963476..964168	+	693	WP_006135935.1	hypothetical protein	-
F6Y32_RS05060	964580..966031	+	1452	WP_006135932.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	955250..969347	14097
	-	flank	IS/Tn	-	-	962717..963085	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9162.82 Da
• Isoelectric Point: 10.3158

>T140727 WP_002966807.1 NZ_CP044340:c961876-961631 [Brucella melitensis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Nucleotide

• Length: 246 bp

>T140727 NZ_CP059386:c3297055-3296753 [Acinetobacter baumannii]
ATGTACTCAATATATACCACAGAAGCCTTTGATGATTGGTTTACCAAGTTAAAAGATCAACAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGGAACTTTGGAGATACTGAACCAGTCGGTGAAGGTGTTTCTGAGTTACGTTTCT
TCTTTGGACCTGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTCGTTATTCTCTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTACAATTAGCACAAGATTTAGAGGAGGAGCTGTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11653.48 Da
• Isoelectric Point: 10.5824

>AT140727 WP_004683740.1 NZ_CP044340:c961631-961317 [Brucella melitensis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT140727 NZ_CP059386:c3296752-3296471 [Acinetobacter baumannii]
ATGGCTATCAAACTTCGTAAATGGGATAGTGCTGAGCATCTTAAAACAGAAGAAGATATGCAAGCTTACCTACAAGCCTG
TATCGATGAATCTAATGGTGATGCTGCATTCATTGCTAAAGCTTTAGGTAACATTGCTAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGTCTAGGCCGAGAAAGTCTTTACAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Structures

Toxin

• AlphaFold DB: A0A7U8PD14

Antitoxin

• AlphaFold DB: C0RIV1