Detailed information of TA system TA140725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 18632..19429
• Replicon: plasmid unnamed1
• Accession: NZ_CP044336
• Organism: Enterobacter hormaechei strain AKB48

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: D6T76_RS22030
• Protein ID: WP_169724906.1
• Coordinates: 18632..19135 (-)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A5D4NAG3
• Locus tag: D6T76_RS22035
• Protein ID: WP_016582626.1
• Coordinates: 19160..19429 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D6T76_RS22000	15734..16237	+	504	Protein_8	tail fiber domain-containing protein	-
D6T76_RS22005	16302..17090	+	789	WP_169724838.1	receptor-recognizing protein	-
D6T76_RS22010	17165..17488	+	324	WP_000064173.1	hypothetical protein	-
D6T76_RS22015	17502..18194	+	693	WP_169724839.1	hypothetical protein	-
D6T76_RS22020	18196..18447	+	252	WP_000147960.1	hypothetical protein	-
D6T76_RS22025	18398..18604	+	207	WP_080332520.1	hypothetical protein	-
D6T76_RS22030	18632..19135	-	504	WP_169724906.1	GNAT family N-acetyltransferase	Toxin
D6T76_RS22035	19160..19429	-	270	WP_016582626.1	DUF1778 domain-containing protein	Antitoxin
D6T76_RS22040	19846..20511	+	666	Protein_16	AAA family ATPase	-
D6T76_RS22045	20517..20870	+	354	WP_160859104.1	hypothetical protein	-
D6T76_RS22050	20912..21709	-	798	WP_169724840.1	DUF2971 domain-containing protein	-
D6T76_RS22055	21973..22698	+	726	WP_006812583.1	hypothetical protein	-
D6T76_RS22060	22759..24099	+	1341	WP_169724841.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..126563	126563

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (39-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18645.29 Da
• Isoelectric Point: 8.4206

>T140725 WP_169724906.1 NZ_CP044336:c19135-18632 [Enterobacter hormaechei]
MFSAQAVYDFSGFDCGEPSLNDFLQNRPVQQHNGRILRGYLLLTKDPVPKVKGFYTLSGSCFARQTLPSNTQKRKIPYAD
APSVTLGRLAIDISLQKQGYGEVLVTHALKVVYQASRAVGIYALFVDALNQSAMQFYQKFDFIPLTGANANSLFYPTKSI
EELFETE

Nucleotide

• Length: 504 bp

>T140725 NZ_CP059386:c3295727-3295431 [Acinetobacter baumannii]
ATGATTGAAATTAAACGTCTACCAGAATTTGATGAATGGTTGGATGGCATTAAAGACAATATGACCCGCATTCGCTTGAA
TCGCCGATTAGATAAGGTTCAACGCGGGAACTGGGGAGACATTAAACCTCTCCAAGATGGTGTTTGGGAAATGAGAGAGT
TCTTCGGTTCAGGATGGAGGATGTATTACATCCAACATGGTGACGTAGTGATTGTAATGCTTGGTGGCGGAGATAAATCA
ACTCAACAACAAGACATCGATCGTGCAGTCAAATTATCGAAAACGTTGGAGGATTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10113.57 Da
• Isoelectric Point: 5.8580

>AT140725 WP_016582626.1 NZ_CP044336:c19429-19160 [Enterobacter hormaechei]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNLVMDAISNPPAPNDRLKR
AAKRLQSME

Nucleotide

• Length: 270 bp

>AT140725 NZ_CP059386:c3295429-3295139 [Acinetobacter baumannii]
ATGGTTAAAGTAGCTGATTTACCAAGTTTTGATATGGCTGAGTCACTCAAAACTGAAGAAGACATCGTGATGTATCTCAA
TATGGTTCTTGAAGATAATGATCCTGCTGAATTAGCTCATGCCCTTGGTGTCATTGCTAAAGCTAGAGGTATGACCCAAA
TTGCTAAAGAAGCAGGGATCGGACGTGAAGCACTTTATAAAGCACTTCGCCATGATTCAGCACCACGTTTTGATACGATT
AATCGTGTAGTAAATGCTTTAGGCTTAAAACTAAGAGTGCAACACGCCTAA

Structures

Antitoxin

• AlphaFold DB: A0A5D4NAG3