Detailed information of TA system TA140723

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 4174106..4174726
• Replicon: chromosome
• Accession: NZ_CP044335
• Organism: Enterobacter hormaechei strain AKB48

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A837FFM2
• Locus tag: D6T76_RS19695
• Protein ID: WP_015571250.1
• Coordinates: 4174106..4174324 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: D6T76_RS19700
• Protein ID: WP_169724376.1
• Coordinates: 4174352..4174726 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D6T76_RS19665	4170118..4170378	+	261	WP_015571255.1	type B 50S ribosomal protein L31	-
D6T76_RS19670	4170381..4170521	+	141	WP_003859006.1	type B 50S ribosomal protein L36	-
D6T76_RS19675	4170518..4171228	-	711	WP_169724374.1	GNAT family N-acetyltransferase	-
D6T76_RS19680	4171330..4172790	+	1461	WP_169724821.1	PLP-dependent aminotransferase family protein	-
D6T76_RS19685	4172762..4173229	-	468	WP_169724375.1	hypothetical protein	-
D6T76_RS19690	4173346..4173897	-	552	WP_077579932.1	maltose O-acetyltransferase	-
D6T76_RS19695	4174106..4174324	-	219	WP_015571250.1	hemolysin expression modulator Hha	Toxin
D6T76_RS19700	4174352..4174726	-	375	WP_169724376.1	Hha toxicity modulator TomB	Antitoxin
D6T76_RS19705	4175237..4178383	-	3147	WP_169724377.1	multidrug efflux RND transporter permease subunit AcrB	-
D6T76_RS19710	4178406..4179599	-	1194	WP_169724378.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8583.95 Da
• Isoelectric Point: 8.9008

>T140723 WP_015571250.1 NZ_CP044335:c4174324-4174106 [Enterobacter hormaechei]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Nucleotide

• Length: 219 bp

>T140723 NZ_CP059386:c1448122-1447859 [Acinetobacter baumannii]
ATGCATGTATTTACATATACAGACGCACGTAACAACTTAAAATCTGTTTTAGATAAAGTTATTGATGATGCAGATGTCGC
TTTCATTACAAGAAAAGAAGGCGGTCATGCTGTAGTAATGGGACAAGATCATTACGATAGTTTAATGGAAACGTTGTATC
TCCTTTCATCTCCAAATAATACCACTCGTTTAAACGAATCAATTGCTCAATTACGCGCAGGTAAAACCAAACAAAGAGAG
TTGATTAAAGATGACGAGTCGTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14485.22 Da
• Isoelectric Point: 4.8989

>AT140723 WP_169724376.1 NZ_CP044335:c4174726-4174352 [Enterobacter hormaechei]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYT
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Nucleotide

• Length: 375 bp

>AT140723 NZ_CP059386:c1447872-1447609 [Acinetobacter baumannii]
ATGACGAGTCGTAATGTCGAATGGACTGAAAATGCCTGGGATGAATATATCTATTGGCAAACACAGGATAAAAAGATACT
CAAACGTATCAATACCTTAATCAGAGAATGTCAAAGAACACCCTTTGAAGGAACTGGGAAACCAGAACCTTTAAAAGCAA
ATCTTTCAGGATTCTGGAGCCGTAGAATTGATGAGAAGCATAGATTAGTTTATGAAGTGACGGATGAACGAATTTCCATT
ATTCAATGTCGGTTCCATTACTAA

Structures

Toxin

• AlphaFold DB: A0A837FFM2