Detailed information of TA system TA140719

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2784286..2784902
• Replicon: chromosome
• Accession: NZ_CP044335
• Organism: Enterobacter hormaechei strain AKB48

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D6T76_RS13035
• Protein ID: WP_150390848.1
• Coordinates: 2784286..2784657 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: D6T76_RS13040
• Protein ID: WP_015569912.1
• Coordinates: 2784660..2784902 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D6T76_RS13020	2781786..2782688	+	903	WP_169723473.1	formate dehydrogenase subunit beta	-
D6T76_RS13025	2782685..2783320	+	636	WP_003861958.1	formate dehydrogenase cytochrome b556 subunit	-
D6T76_RS13030	2783317..2784246	+	930	WP_169723474.1	formate dehydrogenase accessory protein FdhE	-
D6T76_RS13035	2784286..2784657	-	372	WP_150390848.1	PIN domain-containing protein	Toxin
D6T76_RS13040	2784660..2784902	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
D6T76_RS13045	2785101..2786021	+	921	WP_169723475.1	alpha/beta hydrolase	-
D6T76_RS13050	2786030..2786971	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
D6T76_RS13055	2787016..2787453	-	438	WP_015569909.1	D-tyrosyl-tRNA(Tyr) deacylase	-
D6T76_RS13060	2787450..2788331	-	882	WP_169723476.1	virulence factor BrkB family protein	-
D6T76_RS13065	2788325..2788924	-	600	WP_003861947.1	glucose-1-phosphatase	-
D6T76_RS13070	2789043..2789843	-	801	WP_169723477.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13707.85 Da
• Isoelectric Point: 6.7561

>T140719 WP_150390848.1 NZ_CP044335:c2784657-2784286 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRIEAADAIEHTASHRAISLITRMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T140719 NZ_CP059383:c238543-238208 [Streptococcus agalactiae]
TTGGACTATAAGAAATATCAGATTATCTATGCTCCTGATGTTTTAGAGAAGCTAAAAGAAATTCGTGATTATATTTCCCA
AAACTATTCCTCAACATCAGGTCAGCGTAAAATGGAGCAAATCATTAATGACATAGAAAAACTTGAAGTTTTCCCAGAAG
TTGGTTTTGATGCCGATGAAAAATATGGTTCAGAAATCAGCAATTATCACAGTACTAGAGGTTACACCTTAAGTAAAGAT
TATATTGTCCTATACCATATCGAAGAGGAAGAGAATAGGGTTGTCATTGATTACTTGCTTCCTACTCGAAGCGACTATAT
GAAATTGTTTAAATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT140719 WP_015569912.1 NZ_CP044335:c2784902-2784660 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT140719 NZ_CP059383:c238820-238533 [Streptococcus agalactiae]
ATGGTTACAGTAGAAAAAAATAGAGCTGTCACATTCCAAGCTAACAAAGAATTGGTAAGCGAAGCAATGACAGTATTAAA
CAAAAAAAATTTAACCTTATCATCTGCTTTAAGATTATTCCTACAAAATGTCGTTGTCACAAACGAGGTTGACTTATTGA
CGGAAGAGGAGTTAGAGAAAGAAAAACTTTTCAAGCAATTTCAAGCCGAAATCAGCAAAAATATTGAGGATGTTCGTCAA
GGGAAGTTTTATACCTCTGAAGAAGTGAGGGCTGAACTTGGACTATAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3