Detailed information of TA system TA140718

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 2683765..2684361
• Replicon: chromosome
• Accession: NZ_CP044335
• Organism: Enterobacter hormaechei strain AKB48

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: D6T76_RS12605
• Protein ID: WP_169723408.1
• Coordinates: 2683765..2684067 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: D6T76_RS12610
• Protein ID: WP_032672198.1
• Coordinates: 2684074..2684361 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D6T76_RS12585	2679984..2681333	+	1350	WP_169723406.1	lysine-sensitive aspartokinase 3	-
D6T76_RS12590	2681427..2682371	+	945	WP_023295757.1	ketopantoate/pantoate/pantothenate transporter PanS	-
D6T76_RS12595	2682422..2682694	+	273	WP_017382612.1	DUF3811 domain-containing protein	-
D6T76_RS12600	2682695..2683567	-	873	WP_169723407.1	23S rRNA pseudouridine(2604) synthase RluF	-
D6T76_RS12605	2683765..2684067	+	303	WP_169723408.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D6T76_RS12610	2684074..2684361	+	288	WP_032672198.1	putative addiction module antidote protein	Antitoxin
D6T76_RS12615	2684358..2685989	-	1632	WP_169723409.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11455.27 Da
• Isoelectric Point: 10.3751

>T140718 WP_169723408.1 NZ_CP044335:2683765-2684067 [Enterobacter hormaechei]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRVYFKDQGNCIIVLLCGGDKS
SRARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T140718 NZ_CP059377:c314035-313691 [Ralstonia solanacearum]
ATGGATGCTACCTTTATCGAGTTGCCGCCTTTTCAGAGGCTGCGGGAGCACTATCTCTCGGACGACGAGTACCGAGCGCT
CCAGCAGGAACTGCTGGCCCGGCCGGACGCCGGCGACGTCATCAAGGGGACAGGTGGCCTACGCAAGCTGCGATTTTCGG
ACAAGCGGCGCGGCAAAGGTAAGCGCGGCGGTCTGCGCGTGATTTACTACCACTGGGATGGAGGCGGCCAATTCTGGATG
TTTGTCGTCTACGACAAGGGCGAGGCGGACGACCTTTCCAGCGACGAGCGCAAAGTGCTGGCACGGCTGCTGGAGCAGGA
AGTCAAGGCAAGGAGTGAGCGATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10088.51 Da
• Isoelectric Point: 4.7045

>AT140718 WP_032672198.1 NZ_CP044335:2684074-2684361 [Enterobacter hormaechei]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAEALGVIARAKGMSTISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPSGD

Nucleotide

• Length: 288 bp

>AT140718 NZ_CP059377:c313694-313383 [Ralstonia solanacearum]
ATGAAACGGAACCTGTTTGCTGAGTTGAAGGAGGGGATGGATGCGCTTGCCGCAGAGCGCGAAGGAAAGATGACCCTTCG
CAAGGTGAAGGTCCGCGTGCTCCAGCCGCTTGACGTGACAGCCGAAGAAATCAAGGCCGTTCGCGCAGCGGCACATGCTT
CACAGGCTGTCATGGCACGTCGACTGCGCGTCAATGTGCGCACCTACCAGAACTGGGAACAAGGAACCGCCAAACCGAAC
ACCCAGGCGGCTGTGTTGATCAAGCTCGTCGAGAAGCATCCGGAAACGTTGCAGATGCTGGAAGCGCTTTAA