Detailed information of TA system TA140701

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1212..1846
• Replicon: plasmid unnamed1
• Accession: NZ_CP044332
• Organism: Methylocystis parvus strain BRCS2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: F7D14_RS19555
• Protein ID: WP_016919208.1
• Coordinates: 1212..1613 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6B8M6T5
• Locus tag: F7D14_RS19560
• Protein ID: WP_026016140.1
• Coordinates: 1610..1846 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D14_RS19550	728..1105	+	378	WP_016919209.1	single-stranded DNA-binding protein	-
F7D14_RS19555	1212..1613	-	402	WP_016919208.1	type II toxin-antitoxin system VapC family toxin	Toxin
F7D14_RS19560	1610..1846	-	237	WP_026016140.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
F7D14_RS19565	2023..2406	-	384	WP_154420362.1	hypothetical protein	-
F7D14_RS19570	2438..3124	-	687	WP_016919205.1	P-loop NTPase	-
F7D14_RS19575	3206..3490	+	285	WP_154420364.1	hypothetical protein	-
F7D14_RS19580	4170..4574	+	405	WP_016919203.1	plasmid mobilization relaxosome protein MobC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..248223	248223

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (10-54)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14467.87 Da
• Isoelectric Point: 7.8528

>T140701 WP_016919208.1 NZ_CP044332:c1613-1212 [Methylocystis parvus]
MTRYLLDTNIISDLIRNPKGKVAKHIARVGEKNVCTSIIVAAELRYGCAKSGSKRLLEAVELLLGELDVLSLEAPADAEY
GRIRAELERKGSPIGGNDLLIAAHALAIEATMVTANVDEFTRVKGLKVQNWLL

Nucleotide

• Length: 402 bp

>T140701 NZ_CP059348:c3778180-3777773 [Providencia rettgeri]
ATGATTAAATACTTGCTTGATACTAATATTATTATTTTCACCATCAAACGACGACCTGCAGCATTATTACCAAAGTTTAA
CCAGCATGCTGACCAATTGGCAATTTCAACAATTACGTTAGCAGAGCTGGTTTTTGGCGCAGAAAAAAGCATGAATCCAG
AACGAAATTTAGCTGTTGTTAATGATTTCGTTTCTCGGCTTTGCGTGTTGCCTTATGATGAAGCGGCCGCTTGTCATTAC
GGTGATATTCGAGCAAATCTCGAAAAACAAGGTAAAAAAATCGGGGAAAATGATCTACACATAGCGGCTCACGCTAGAAG
CAAAGGGCTTATCGTCGTCACAAATAATACTCGCGAGTTTGAAAGGGTTGATGGTTTAAGATTAGAGGATTGGGTCACTT
CATCATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9024.39 Da
• Isoelectric Point: 4.9062

>AT140701 WP_026016140.1 NZ_CP044332:c1846-1610 [Methylocystis parvus]
MGEARHVKLFRNGRNQAVRIPVEFELPGDEAIMRREGDRLVLEPTRKRGLLGLLETMEPLDETFPEIEDRPPAPEKIF

Nucleotide

• Length: 237 bp

>AT140701 NZ_CP059348:c3778412-3778173 [Providencia rettgeri]
ATCCTACACAGTAAGGTTTTTATGAGTAATCGGACTCAAAATGTTCGTTTACCCGCAGAGTTACGGTTTCCTGATGATGT
GAAAGAAGTCACCGTTAGAATGAAAGGTAAAGAGCGTATTATTACACCACTGCAAAATACGTGGGATAGCTTCTTTTTAA
CGGAAACTTCGGCCACGGATGATTTTTTAGTTTCGAGAGCAGAGCAAGTTCCTACCCCAAGAGAATCTTTCGATGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A6B8M6T5