Detailed information of TA system TA140699

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1355137..1355915
• Replicon: chromosome
• Accession: NZ_CP044331
• Organism: Methylocystis parvus strain BRCS2

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: F7D14_RS06450
• Protein ID: WP_154420200.1
• Coordinates: 1355137..1355565 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A6B8M2P1
• Locus tag: F7D14_RS06455
• Protein ID: WP_016920154.1
• Coordinates: 1355634..1355915 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D14_RS06420	1350236..1350787	+	552	WP_016920161.1	TIGR02301 family protein	-
F7D14_RS06425	1350867..1351121	+	255	WP_016920160.1	hypothetical protein	-
F7D14_RS06430	1351269..1352366	+	1098	WP_016920159.1	histidine phosphatase family protein	-
F7D14_RS06435	1352408..1353718	+	1311	WP_016920158.1	Nramp family divalent metal transporter	-
F7D14_RS06440	1353785..1354075	+	291	WP_016920157.1	hypothetical protein	-
F7D14_RS06445	1354187..1355140	+	954	WP_016920156.1	J domain-containing protein	-
F7D14_RS06450	1355137..1355565	-	429	WP_154420200.1	GNAT family N-acetyltransferase	Toxin
F7D14_RS06455	1355634..1355915	-	282	WP_016920154.1	DUF1778 domain-containing protein	Antitoxin
F7D14_RS06460	1356238..1357533	+	1296	WP_016920153.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
F7D14_RS06465	1357678..1358517	-	840	WP_016920152.1	uracil-DNA glycosylase	-
F7D14_RS06470	1358638..1359498	-	861	WP_016920151.1	AraC family transcriptional regulator	-
F7D14_RS06475	1359609..1359827	-	219	WP_016920150.1	YbdD/YjiX family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (16-120)

Antitoxin

• Vibrio phage ICP1, Orf50 (13-87)

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15253.64 Da
• Isoelectric Point: 9.6237

>T140699 WP_154420200.1 NZ_CP044331:c1355565-1355137 [Methylocystis parvus]
MDEWLKKRARPNQASGASRTYVVCNDETIVAYFALASGAVDLAEATNRLKRNMPDPVPVPVAVLGRLAIDRSWQKQGLGR
ALAKDAFQRVLAASNAIGIRGMLVHAISAEAAAFYQATGFTPSPLDPMTLMATLIDLRAALD

Nucleotide

• Length: 429 bp

>T140699 NZ_CP059348:c1659820-1659299 [Providencia rettgeri]
GTGAAGGACTTTAAAATCGCGATGTATTCAGAGGAAATCGACTATGATTTAACACATTTTGATTGCGGTGAGCCCTATAT
GAATACGTTTCTCACCGAACATTTAAAACGGCAGCATAAAAACCAAATTTTACGTGGGTATATGCTGTTAACCAAAGAAG
AAAAGCCGCGTGTAATGGGCTACTACACCTTATCAGGGAGTAGTTTTGAAAAAAGAATGTTGCCAAGCCGCCAGCGACAA
ATAGCAGTGCCTTACGAAAATGCGCCCAGTGTCACGCTCGGTCGTTTGGCCATTGATAAAAGTATTCAACGACAAGGTTA
TGGTGAGTTACTCGTTGTTCATGCAATGAGAATTGTCTATCAAGCATCGCATGCAGTGGGTATTTACGGCCTATTTGTCG
AGGCCTTAAATGATAAAGCCAAACAATTTTATCTCAGACTCGGTTTTATCCAATTAACAGGAGATAACGCTGATAGCTTT
TTTTATCCAATAAAGTCCATTGAAGCGCTTTTTGATATGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10618.11 Da
• Isoelectric Point: 11.5708

>AT140699 WP_016920154.1 NZ_CP044331:c1355915-1355634 [Methylocystis parvus]
MQSRSNTAKRDTLNLRVKPGDRELIDRAATLVGKSRTDFLLDAGRRAAQDALLDRRLWRVDSAAFDAFVALLDAPPQPNE
RLRRTMKTRAPWE

Nucleotide

• Length: 282 bp

>AT140699 NZ_CP059348:c1660093-1659821 [Providencia rettgeri]
ATGTTAGCCCCCAAAACCAAACGAATCGACTTAAGACTTAATGAAGAGGACAAAAATTTGATTGAAGAAGCTGCAGCAAT
TACCAACCTGTCTATATCTCAGTTCATGATTAGTATTGCATCAGAACGAGCCAGTGAAATTGTCAATCACCATCGACGCC
TCATCCTGAATGAGCATTCATGGAATTTAGTTCAAAATGCTATCAATAACCCACCAACTGTAAATAACCGCTTGAAGCAA
GTTGCCAAACGTCTACAAGACACGGAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A6B8M2P1