Detailed information of TA system TA140698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1215183..1215853
• Replicon: chromosome
• Accession: NZ_CP044331
• Organism: Methylocystis parvus strain BRCS2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: F7D14_RS05770
• Protein ID: WP_016918545.1
• Coordinates: 1215183..1215590 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6B8M3R6
• Locus tag: F7D14_RS05775
• Protein ID: WP_016918546.1
• Coordinates: 1215599..1215853 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D14_RS05740	1210913..1211506	-	594	WP_016918539.1	J domain-containing protein	-
F7D14_RS05745	1211640..1211936	+	297	WP_016918540.1	BolA family transcriptional regulator	-
F7D14_RS05750	1211953..1212924	-	972	WP_016918541.1	alpha/beta fold hydrolase	-
F7D14_RS05755	1212956..1213249	-	294	WP_016918542.1	hypothetical protein	-
F7D14_RS05760	1213403..1213867	+	465	WP_026016048.1	hypothetical protein	-
F7D14_RS05765	1214020..1215186	+	1167	WP_016918544.1	phosphoserine transaminase	-
F7D14_RS05770	1215183..1215590	-	408	WP_016918545.1	type II toxin-antitoxin system VapC family toxin	Toxin
F7D14_RS05775	1215599..1215853	-	255	WP_016918546.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
F7D14_RS05780	1215935..1217521	+	1587	WP_016918547.1	phosphoglycerate dehydrogenase	-
F7D14_RS05785	1217704..1218315	+	612	WP_016918548.1	L,D-transpeptidase	-
F7D14_RS05790	1218397..1218945	-	549	WP_016918549.1	alpha/beta hydrolase	-
F7D14_RS05795	1218945..1220639	-	1695	WP_040578912.1	lysine--tRNA ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-126)

Antitoxin

• SpoVT-AbrB domain (7-43)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14766.90 Da
• Isoelectric Point: 6.4730

>T140698 WP_016918545.1 NZ_CP044331:c1215590-1215183 [Methylocystis parvus]
MFCLDTNVVIFALNGRRAGYAERLAEEIRAGTKLIVPSVVLFELSYGIAKSRQPEKARALLVEFLSAGFEQPAFDADDAH
EAGDIRAHLEARGAPIGPFDVLIAAQARRRGAVLVTNNRREFDRVPGLMATDWAA

Nucleotide

• Length: 408 bp

>T140698 NZ_CP059348:c1659820-1659299 [Providencia rettgeri]
GTGAAGGACTTTAAAATCGCGATGTATTCAGAGGAAATCGACTATGATTTAACACATTTTGATTGCGGTGAGCCCTATAT
GAATACGTTTCTCACCGAACATTTAAAACGGCAGCATAAAAACCAAATTTTACGTGGGTATATGCTGTTAACCAAAGAAG
AAAAGCCGCGTGTAATGGGCTACTACACCTTATCAGGGAGTAGTTTTGAAAAAAGAATGTTGCCAAGCCGCCAGCGACAA
ATAGCAGTGCCTTACGAAAATGCGCCCAGTGTCACGCTCGGTCGTTTGGCCATTGATAAAAGTATTCAACGACAAGGTTA
TGGTGAGTTACTCGTTGTTCATGCAATGAGAATTGTCTATCAAGCATCGCATGCAGTGGGTATTTACGGCCTATTTGTCG
AGGCCTTAAATGATAAAGCCAAACAATTTTATCTCAGACTCGGTTTTATCCAATTAACAGGAGATAACGCTGATAGCTTT
TTTTATCCAATAAAGTCCATTGAAGCGCTTTTTGATATGTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9458.76 Da
• Isoelectric Point: 4.6437

>AT140698 WP_016918546.1 NZ_CP044331:c1215853-1215599 [Methylocystis parvus]
MATAKLFQHGRSQAVRLPKEFRLPGTEVRVSKVGDKVILEPLESEPFDVDAWRLRLDALGAKDFLPEGIPDDQPLRPDES
ISFD

Nucleotide

• Length: 255 bp

>AT140698 NZ_CP059348:c1659155-1658919 [Providencia rettgeri]
ATGACGGTAGCCAATAAAATGAAAAAGACGGTGAGTGTGACAGTCTCCCCTGAACTTTATGAACAAGCGAGAAAAGCCAA
TTTAAATTTCTCCCTCGTGTTATCCAATGCTTTACTGGCTGAACTCAAAAAACTTGAGGCGTTAAAATGGAAAGCGGATA
ACCAAGATGGTTTTCAGGAACTCAATAGGATCAGTGAAGAATATGGAATTTTTTCAGAGGAATATAAGGAGCTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A6B8M3R6