Detailed information of TA system TA140688

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 16809..17328
• Replicon: plasmid p15-307-1_01
• Accession: NZ_CP044317
• Organism: Enterococcus faecium strain 15-307-1

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: CWC51_RS13935
• Protein ID: WP_000588503.1
• Coordinates: 17071..17328 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: CWC51_RS13930
• Protein ID: WP_000388479.1
• Coordinates: 16809..17078 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWC51_RS13885	12006..12905	+	900	WP_002303312.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
CWC51_RS13890	12908..13393	+	486	WP_002303313.1	single-stranded DNA-binding protein	-
CWC51_RS13895	13534..13737	-	204	WP_002316074.1	putative holin-like toxin	-
CWC51_RS13900	13812..14072	+	261	WP_002295623.1	hypothetical protein	-
CWC51_RS13905	14509..14715	+	207	WP_002347218.1	hypothetical protein	-
CWC51_RS13910	14715..14966	+	252	WP_002296238.1	hypothetical protein	-
CWC51_RS13915	14981..15418	+	438	WP_002300851.1	hypothetical protein	-
CWC51_RS13920	15411..16118	+	708	WP_002303117.1	hypothetical protein	-
CWC51_RS13925	16499..16678	+	180	WP_001120991.1	hypothetical protein	-
CWC51_RS13930	16809..17078	+	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CWC51_RS13935	17071..17328	+	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
CWC51_RS13940	17831..18790	+	960	WP_002287225.1	IS30 family transposase	-
CWC51_RS13945	18852..19007	+	156	WP_002347142.1	hypothetical protein	-
CWC51_RS13950	19031..19855	+	825	WP_001809248.1	hypothetical protein	-
CWC51_RS13955	20020..20634	-	615	WP_001261742.1	recombinase family protein	-
CWC51_RS13960	20872..21294	+	423	WP_002303116.1	hypothetical protein	-
CWC51_RS13965	21304..21507	+	204	WP_002299573.1	hypothetical protein	-
CWC51_RS13970	21718..22302	+	585	WP_002299575.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-aph(2'')	-	1..199401	199401

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T140688 WP_000588503.1 NZ_CP044317:17071-17328 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T140688 NZ_CP059347:c1010192-1009989 [Providencia rettgeri]
ATGACCAAATTTGATTATCTGATGCGTTTAAGAAAATGTACAACAATTGAAACTCTTGAGCGTGTCATTGAAAAAAACAA
GTATGAATTATCTGATGATGAGCTAGAGCTATTCTACTCCGCAGCAGATCACCGCTTAGCTGAACTCACAATGAATAAGC
TTTATGATAAAATCCCAGCTTCTGTTTGGAAGTTTGTTAGATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT140688 WP_000388479.1 NZ_CP044317:16809-17078 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT140688 NZ_CP059347:c1010635-1010267 [Providencia rettgeri]
ATGGATGAATACTCACCCAAAAAACATGATATAGCAGAGCTAAAATACTTATGTAATAGCCTCAATAGAGATGCTATTTT
AAGCTTACAAAAAACAAATACACACTGGATTAATGACTTAAGCTCCCCTCAAAGCGCAGCCTTGAATGAGCTTATTGAAC
ATATCGCTGCATTTGTATGGCGGTTTAAAATCAAATATCCAAAAGAGAATCTTGTTATATCACTTATCGAAGAGTACCTC
GATGAGACCTATGACTTATTCGGTAGCCCGGTCATCACGCTCAGTGAAGTTATAGACTGGGAAGCAATGAACCAGAGTCT
TGTCGCTGTGCTGGATGATGACCTAAAATGTCTAACCAGCAAAACCTAA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7