Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 532912..533483 Replicon chromosome
Accession NZ_CP044316
Organism Enterococcus faecium strain 15-307-1

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag CWC51_RS02555 Protein ID WP_002286801.1
Coordinates 533142..533483 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag CWC51_RS02550 Protein ID WP_002323011.1
Coordinates 532912..533142 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CWC51_RS02520 528048..529376 + 1329 WP_008265691.1 FAD-containing oxidoreductase -
CWC51_RS02525 529398..530024 + 627 WP_002286816.1 cysteine hydrolase -
CWC51_RS02530 530207..530788 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
CWC51_RS02535 531520..531867 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
CWC51_RS02540 531843..532082 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
CWC51_RS02545 532287..532625 - 339 WP_002286804.1 hypothetical protein -
CWC51_RS02550 532912..533142 + 231 WP_002323011.1 hypothetical protein Antitoxin
CWC51_RS02555 533142..533483 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CWC51_RS02560 534333..534518 + 186 WP_002304207.1 hypothetical protein -
CWC51_RS02565 535023..535217 + 195 WP_002297028.1 hypothetical protein -
CWC51_RS02570 535375..535657 + 283 Protein_510 transposase -
CWC51_RS02575 535901..536731 - 831 WP_002286789.1 manganese catalase family protein -
CWC51_RS02580 536939..538273 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T140684 WP_002286801.1 NZ_CP044316:533142-533483 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T140684 NZ_CP059346:c819402-819199 [Providencia alcalifaciens]
ATGACCAAATCCGATTATCTGATGCGTTTAAGAAAATGCACCACTATTGAAACACTTGAGCGCGTTATTGAAAAAAATAA
GTATGAGTTATCCGATGATGAGTTAGAGTTATTTTACTCTGCCGCTGATCATCGTCTTGCAGAGCTCACGATGAATAAAC
TTTACGATAAAATTCCAGCATCTGTTTGGAAGTTTGTTAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT140684 WP_002323011.1 NZ_CP044316:532912-533142 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT140684 NZ_CP059346:c819843-819475 [Providencia alcalifaciens]
ATGGATGAATACTCACCCAAGAAACATGATATAGCCGAATTAAAATATCTTTGTAACAGTTTGAATAGAGACGCTATATC
AAGTTTACAAAAAACAAACACTCATTGGATAAATGATCTTAGTTCTTCACAAAGCATCAGTCTTAACGAGCTTGTTGAGC
ATATTGCAGCTTTTGTCTGGCGATTTAAAATCAAATATCCAAAAGAAAATCTCGTTATTTCTCTTGTTGAAGAGTATCTT
GACGAAACATATAATTTATTTGGCAGCCCAGTAGTTACCTTCAGCGAAATCATTGACTGGGAAAGTATGAATCAAAATCT
TGTCGCCGTGCTCGATGATGATCTAAAATGTCTGACAAGTAAGACTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References