Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5434139..5434636 Replicon chromosome
Accession NZ_CP044315
Organism Escherichia coli strain SJ7

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag F7D02_RS27030 Protein ID WP_001173343.1
Coordinates 5434463..5434636 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag F7D02_RS27025 Protein ID WP_000398619.1
Coordinates 5434139..5434435 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D02_RS27005 5430870..5431589 - 720 WP_000611288.1 two-component system response regulator DcuR -
F7D02_RS27010 5431586..5433217 - 1632 WP_001216487.1 sensor histidine kinase -
F7D02_RS27015 5433398..5433628 + 231 WP_000371700.1 (4Fe-4S)-binding protein -
F7D02_RS27020 5433640..5433912 + 273 WP_000405647.1 N-acetyltransferase -
F7D02_RS27025 5434139..5434435 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
F7D02_RS27030 5434463..5434636 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
F7D02_RS27035 5434755..5436272 - 1518 WP_001295074.1 lysine--tRNA ligase -
F7D02_RS27040 5436509..5437966 - 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T140682 WP_001173343.1 NZ_CP044315:5434463-5434636 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T140682 NZ_CP059345:c2296033-2295572 [Providencia rettgeri]
ATGATTTTATATCGTTTGATTAAATCTGACTTTGCTCATGATGCGTGGTCCGGTCAAGGTGCTAGGTTATACGGTGGGCG
CTGGAACCATAAAGGTACGCCCGCTATCTACACATCAACAAGTATTTCATTAGCTGCTTTAGAAATTCTCGTTCATATAA
ACCAAGATACCGTGCTTAATCATTTCACTTTATTATCAATTGAGATCAACGATAGGTATGTGATGAAGCTGTCAGTAGAG
CATCTCCCTGATGATTGGCAGCGTGACCCAGCACCTACCTCCACAATGGACATAGGCACAACCTGGTTAACGAACCAAGA
CAGCCTAGCCTTAATTGTCCCTTCTTGTATTATTCCTTATGAATATAATGCCATTATTAACCCTATGCACCCGCAATTTC
AAAAAGTGACCCAAACCATTAAGCCCCTCGACTTTGCTTTTGACCCACGATTTAACCGGTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT140682 WP_000398619.1 NZ_CP044315:5434139-5434435 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT140682 NZ_CP059345:c2296491-2296030 [Providencia rettgeri]
ATGAGAAACTATATTCCAGCACTCCCAACCCAACCCGCCACGCCACCTTTTAAGCCCCTATGGCGCTTTGCTGGCCTACC
CGCAGAACGTGGTATCGAACTCACTCATTTCCTACGACAAGGTCTTTCCGTTACGGTGTTAGACAACATTCAAGAATGGT
CTGGCATCAGCAAATCTGAATTACTCCGTATTTCAGGTATTAATGAGCGCAATGTGGCAAGACGTAGAAATGCAGGTCAG
CCACTCAATGCCGATGAAAGTGAAAGAATTGCTCGCTTAGTGCGTGTATTTGATGCCGCTGTACGCTTATTCAATGGTGA
TAAGTCAATGGCAAGTGATTGGTTGAATCACCCCGTGAAAGGCTTAGGACATACGCGCCCTATAGAGTTGATTGCCACTG
AAAGTGGTGCTCTTGAAGTTATCGACCTTATTGGCCGCATTGAACACGGTGTTTTTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References