Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4670814..4671445 Replicon chromosome
Accession NZ_CP044315
Organism Escherichia coli strain SJ7

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag F7D02_RS23565 Protein ID WP_001259385.1
Coordinates 4671170..4671445 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag F7D02_RS23560 Protein ID WP_000593554.1
Coordinates 4670814..4671173 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D02_RS23530 4665926..4666399 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
F7D02_RS23535 4666396..4666677 + 282 WP_000084022.1 PTS sugar transporter subunit IIB -
F7D02_RS23540 4666754..4668112 + 1359 WP_001298729.1 PTS galactitol transporter subunit IIC -
F7D02_RS23545 4668105..4669613 + 1509 WP_001298718.1 carbohydrate kinase -
F7D02_RS23550 4669603..4669872 + 270 WP_000933060.1 HPr family phosphocarrier protein -
F7D02_RS23555 4669904..4670764 + 861 WP_001131783.1 class II aldolase -
F7D02_RS23560 4670814..4671173 - 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
F7D02_RS23565 4671170..4671445 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
F7D02_RS23570 4671518..4672642 - 1125 WP_001216257.1 ABC transporter permease -
F7D02_RS23575 4672642..4675378 - 2737 Protein_4618 ribosome-associated ATPase/putative transporter RbbA -
F7D02_RS23580 4675375..4676442 - 1068 WP_000361467.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T140680 WP_001259385.1 NZ_CP044315:c4671445-4671170 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T140680 NZ_CP044315:c4671445-4671170 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT140680 WP_000593554.1 NZ_CP044315:c4671173-4670814 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT140680 NZ_CP044315:c4671173-4670814 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References