Detailed information of TA system TA140677

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4014533..4015331
• Replicon: chromosome
• Accession: NZ_CP044315
• Organism: Escherichia coli strain SJ7

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0F3UPG9
• Locus tag: F7D02_RS20115
• Protein ID: WP_000854698.1
• Coordinates: 4014954..4015331 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0F3T7G0
• Locus tag: F7D02_RS20110
• Protein ID: WP_000017094.1
• Coordinates: 4014533..4014907 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D02_RS20075	4009560..4009718	+	159	WP_001369071.1	DUF905 family protein	-
F7D02_RS20080	4009873..4010253	+	381	Protein_3930	DUF932 domain-containing protein	-
F7D02_RS20085	4010763..4012541	+	1779	WP_001243195.1	reverse transcriptase/maturase family protein	-
F7D02_RS20090	4012660..4013100	+	441	Protein_3932	DUF932 domain-containing protein	-
F7D02_RS20095	4013191..4013676	+	486	WP_000213700.1	antirestriction protein	-
F7D02_RS20100	4013691..4014168	+	478	Protein_3934	RadC family protein	-
F7D02_RS20105	4014237..4014458	+	222	WP_001220316.1	DUF987 domain-containing protein	-
F7D02_RS20110	4014533..4014907	+	375	WP_000017094.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
F7D02_RS20115	4014954..4015331	+	378	WP_000854698.1	TA system toxin CbtA family protein	Toxin
F7D02_RS20120	4015328..4015816	+	489	WP_001054226.1	hypothetical protein	-
F7D02_RS20125	4015828..4016025	+	198	WP_000839251.1	DUF957 domain-containing protein	-
F7D02_RS20130	4016110..4016952	+	843	WP_001280439.1	DUF4942 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	espL2 / nleB1 / nleE / efa1 / efa1 / espK / espF / escG / escF / cesD2 / espB / espD / espA / sepL / escD / eae / cesT / tir / map / cesF / espH / sepQ/escQ / escO / escN / escV / cesL / escI / escJ / sepD / escC / cesD / etgA / escU / escT / escS / escR / escL / escK / cesAB / escE / espG	3970816..4058388	87572

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13975.94 Da
• Isoelectric Point: 7.9087

>T140677 WP_000854698.1 NZ_CP044315:4014954-4015331 [Escherichia coli]
MKTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRSHYRTVNDITLGKRTEAKR

Nucleotide

• Length: 378 bp

>T140677 NZ_CP059343:c2764182-2763928 [Kocuria varians]
ATGCGGCTGGTCTGGGACGAGGCCGCCTGGGAGGACTACACGTACTGGCAGACCGCCGACCGGAGAATTCTTAAGCGAAT
CAACCTGCTCATCGATGCCTGCCTGCGCGAGCCGTTCGAGGGCATCGGCAAGCCCGAGCAGCTGAAATACGGGGCGCACG
GCTCGTGGTCGCGGCGCATCAACGAGGAGCACCGGCTCGTCTACCTCGTGACTGGTGAGGATCTCGTGATCTTGCAGTCT
CGATACCACTACTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13732.42 Da
• Isoelectric Point: 5.4488

>AT140677 WP_000017094.1 NZ_CP044315:4014533-4014907 [Escherichia coli]
VSGTLHETNYPNDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT140677 NZ_CP059343:c2764461-2764183 [Kocuria varians]
ATGTCCATCAGCGCAAGCGAGGCCCGCAAGACTCTCTTCCCGCTCATCGAGCGCGTGAACGAAGACCAGGAAGCCGTCGA
GATTGTCTCTCGGAAGGGCAACGCGGTGCTCATGCCCGCCGACGAGTACGCGGCGTGGCAAGAGACCGCCTACCTGTTCC
GGTCGCCGTCGAACGCCCGCAGGCTGCTCGACGCCTACGATCGTGCCCGTGCCGGGAAAACTGAGGTCCACGAGCTCGAT
CGTTCCGACGAGTCGGTCAGCCAGGCTCGGGACGTGTGA

Structures

Toxin

• AlphaFold DB: A0A0F3UPG9

Antitoxin

• AlphaFold DB: A0A0F3T7G0