Detailed information of TA system TA140675

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 3798742..3799517
• Replicon: chromosome
• Accession: NZ_CP044315
• Organism: Escherichia coli strain SJ7

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: F7D02_RS19070
• Protein ID: WP_001193489.1
• Coordinates: 3798742..3799113 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7V7JFU6
• Locus tag: F7D02_RS19075
• Protein ID: WP_001059301.1
• Coordinates: 3799152..3799517 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D02_RS19055	3794700..3795416	+	717	WP_001295734.1	N-acetylneuraminic acid outer membrane channel NanC	-
F7D02_RS19060	3795436..3796542	+	1107	WP_001298927.1	N-acetylneuraminate epimerase	-
F7D02_RS19065	3796607..3797587	+	981	WP_000991444.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
F7D02_RS19070	3798742..3799113	-	372	WP_001193489.1	TA system toxin CbtA family protein	Toxin
F7D02_RS19075	3799152..3799517	-	366	WP_001059301.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
F7D02_RS19080	3799543..3799764	-	222	WP_000691983.1	DUF987 domain-containing protein	-
F7D02_RS19085	3799761..3800303	-	543	WP_015740440.1	DNA repair protein RadC	-
F7D02_RS19090	3800316..3800759	-	444	WP_001096016.1	antirestriction protein	-
F7D02_RS19095	3800790..3801611	-	822	WP_001234408.1	DUF945 domain-containing protein	-
F7D02_RS19100	3801731..3802204	-	474	WP_001298943.1	hypothetical protein	-
F7D02_RS19105	3802276..3802728	-	453	WP_000734322.1	hypothetical protein	-
F7D02_RS19110	3802764..3803480	-	717	WP_000174910.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimE / fimB	3791568..3806258	14690

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (17-117)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13875.97 Da
• Isoelectric Point: 7.6717

>T140675 WP_001193489.1 NZ_CP044315:c3799113-3798742 [Escherichia coli]
MQTISSHPTRATQPCLSPVEIWQRLLTCLLSQHYGLTLNDTPFSNETTILEHIDAGVSLCDAVNFLVEKYERVRIDCNDC
SVMEKSPFITSIDILRARKASGLMKRNSHKTVTRVTAGRLQEL

Nucleotide

• Length: 372 bp

>T140675 NZ_CP059331:2517356-2517628 [Mannheimia haemolytica]
ATGCGAGAGATTGAGTATAGTAATGCTTTTAAACGTGACTATAAAAAATGTGCGGTTGAGTCTTTATCCAACTCTTCATT
AATTGAGGCGTTGTATTTTCTGATTAATGACAAGCCATTACCTGAGAAATACTGCGATCACGCTTTAACCGGCGAATGGA
AAGGTTTTAGGGATTGCCATATCCAACCTGATTTGGTTCTTATCTATCGAAAAGTGGAAGATGATAAGCTAGAGCTGCTG
CGAATTGGCAGCCACTCTCATTTATTTGGCTAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13388.25 Da
• Isoelectric Point: 5.9530

>AT140675 WP_001059301.1 NZ_CP044315:c3799517-3799152 [Escherichia coli]
MNNHSESGTKPENPACQQWGLKCAITPCFGARLVQEGNRLHFLSDRAGFSGAFAVDVAMRLDQAFPLMMKQLELMLTSGE
LNPRHPHCVTLYHNGLTCEADTLGSCGYVYIAIYPEQTEPQ

Nucleotide

• Length: 366 bp

>AT140675 NZ_CP059331:2517103-2517369 [Mannheimia haemolytica]
ATGGCAAATAGTGCATTAGTAAGCGTAAGAATCAGCCCTGAAATTAAAGAGCAAGCAAGTGAGGTGTTAGCCGGTATCGG
CTTGACTGTCTCTGATGTGATGCGAATGACATTAGCGAAAATCGCAACAGAAAAACGTTTTCAGTTTGATTATCAGCCTA
ATTTTGAGACAGCAGAAGTATTAAGGGCTGTTAAGAATGGCGAGGAAAAGCTACATTCAGCAAATAGTATTGCGGATTTA
ATGGAGCAACTCAATGCGAGAGATTGA

Structures

Antitoxin

• AlphaFold DB: A0A7V7JFU6