Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3182172..3183009 Replicon chromosome
Accession NZ_CP044315
Organism Escherichia coli strain SJ7

Toxin (Protein)


Gene name itaT Uniprot ID A0A0F5GET7
Locus tag F7D02_RS16140 Protein ID WP_000227783.1
Coordinates 3182467..3183009 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag F7D02_RS16135 Protein ID WP_001297137.1
Coordinates 3182172..3182483 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D02_RS16110 3177192..3178139 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
F7D02_RS16115 3178161..3180152 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
F7D02_RS16120 3180142..3180756 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
F7D02_RS16125 3180756..3181085 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
F7D02_RS16130 3181097..3181987 + 891 WP_000971336.1 heme o synthase -
F7D02_RS16135 3182172..3182483 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
F7D02_RS16140 3182467..3183009 + 543 WP_000227783.1 GNAT family N-acetyltransferase Toxin
F7D02_RS16145 3183065..3184000 - 936 WP_001434881.1 sel1 repeat family protein -
F7D02_RS16150 3184408..3185772 + 1365 WP_001000978.1 MFS transporter -
F7D02_RS16155 3185900..3186391 - 492 WP_001138904.1 nucleotide binding protein YajQ -
F7D02_RS16160 3186559..3187470 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19776.96 Da        Isoelectric Point: 8.0330

>T140673 WP_000227783.1 NZ_CP044315:3182467-3183009 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKQGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T140673 NZ_CP059331:1108459-1108755 [Mannheimia haemolytica]
ATGTATATAGATTTGCAATATGCTGTGCCGTTATTGTTTGAATACGACCCGAACAAAAGCCAAATTAATTTAGCCAAATA
TGGTATTGATTTTGAACAGGCAAAGTTGCTTTGGGAAGATGAACGAAAAGTTGTACTGGAGGCTTGCATTGAACCCGAAT
TACGCTATTTCCTGATTGGCAAGATTTATGATAAATATTGGACAGCGTTTTTTACACCAAGAAATGATGTTATTCGTTTA
ATTTCAGTTAGACGTTCCCGTAAAAAAGAGATCTCATACTATGAGAAATACCATTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT140673 WP_001297137.1 NZ_CP044315:3182172-3182483 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT140673 NZ_CP059331:1108739-1108981 [Mannheimia haemolytica]
ATGAGAAATACCATTAGTGCCGAAGAATTAGAACGTATCTTCGATGAAGGCAAAGAAGATATTTTACAATACTGCGATCT
TTCTACCGCTCATCGCCCGGGGCAAATGAAAAGGGTGAATGTTGATTTCCCAAATTGGATGGTAGAAGCGTTAGATAAGC
AAGCCAGACATTTAGGTGTGCCTCGCCAAGCCTTGAGTAAAATGTGGATTGCGGAAAAATTACCGGTTCAATCACCTAGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F5GET7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References