Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3148093..3148711 Replicon chromosome
Accession NZ_CP044315
Organism Escherichia coli strain SJ7

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag F7D02_RS15970 Protein ID WP_001291435.1
Coordinates 3148493..3148711 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag F7D02_RS15965 Protein ID WP_000344800.1
Coordinates 3148093..3148467 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D02_RS15955 3143182..3144375 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
F7D02_RS15960 3144398..3147547 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
F7D02_RS15965 3148093..3148467 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
F7D02_RS15970 3148493..3148711 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
F7D02_RS15975 3148883..3149434 + 552 WP_000102564.1 maltose O-acetyltransferase -
F7D02_RS15980 3149550..3150020 + 471 WP_000136192.1 YlaC family protein -
F7D02_RS15985 3150184..3151734 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
F7D02_RS15990 3151776..3152129 - 354 WP_000878141.1 DUF1428 family protein -
F7D02_RS16000 3152508..3152819 + 312 WP_000409911.1 MGMT family protein -
F7D02_RS16005 3152850..3153422 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T140672 WP_001291435.1 NZ_CP044315:3148493-3148711 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T140672 NZ_CP059331:880856-881131 [Mannheimia haemolytica]
ATGGGTATCACCGAATTTGATGCCGCCGAATACCTCACCAATGACGAGCTAATCGCCCTTTATTTGGCGGAAACATTGAA
AGACGGCACGGACGAGGAGTTTATTCAGGCATTGAATACCGTGGCACGAGCGAAAGGAATGAATGAGTTAGCGAAAAAAG
CAGGGATCGGGCGTGAAAGTTTGTATCAAAGCCTTTCCAGCAGTAAGCCCCGTTTTGAGACTATCCGCAAAATTATTAGC
GCATTAAATTTGGAGTTGTCGGTCGTTAAGGCGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT140672 WP_000344800.1 NZ_CP044315:3148093-3148467 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT140672 NZ_CP059331:880489-880740 [Mannheimia haemolytica]
ATGTACCATATTGAACGCACTGAAATTTTCGACCAATGGCTTGAAAATTTAAAAGATCCACTTGCTGTGGTGACCATTGC
AAGACGTATTGAACGTGCCGAAAGCGGAAATTTTGGCGATACCAAATATATCGCAGAAGGAATTTCCGAAATGAGATTAA
ACGTCAGCAAAGGTTATCGCCTTTACTATGCACAAAAAGGTAGCATTATTTATATACTACTAAATGGAGGATATAAAGGC
ACGCCAAGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References