Detailed information of TA system TA140659

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 630974..631599
• Replicon: chromosome
• Accession: NZ_CP044315
• Organism: Escherichia coli strain SJ7

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: F7D02_RS03090
• Protein ID: WP_000911330.1
• Coordinates: 631201..631599 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: F7D02_RS03085
• Protein ID: WP_000450524.1
• Coordinates: 630974..631201 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D02_RS03060	626777..627247	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
F7D02_RS03065	627247..627819	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
F7D02_RS03070	627965..628843	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
F7D02_RS03075	628860..629894	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
F7D02_RS03080	630107..630820	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
F7D02_RS03085	630974..631201	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
F7D02_RS03090	631201..631599	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
F7D02_RS03095	631746..632609	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
F7D02_RS03100	632624..634639	+	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
F7D02_RS03105	634713..635411	+	699	WP_000679812.1	esterase	-
F7D02_RS03110	635521..635721	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T140659 WP_000911330.1 NZ_CP044315:631201-631599 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T140659 NZ_CP059329:c2186935-2186711 [Mannheimia haemolytica]
ATGGTAGTAAAAATCCTAAAGCGGAAATCTCAAAAGACACTACAACAAATCTACACCCAGCCTATTTCTGCCAATATTAA
ATGGACAAATATAGAAGCTCTTTTTCTAGAGTTAGGAGCAGAAATAGAAGAACGAGAAGGGAGCAGAATTGCAGTTGTTT
TATTTCAACAGGTCAGAGTATTTCATCGCCCACACCCGAATCCTGATACAGATAAGGGGCGGTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT140659 WP_000450524.1 NZ_CP044315:630974-631201 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT140659 NZ_CP059329:c2186668-2186339 [Mannheimia haemolytica]
ATGCGTAATATTATGGATATTAATGGTTATAAAGCTGTCATTGCTTACGATCCCGAAACAGAATTATTTCGAGGTGAATT
TATCGGATTAAATGGTGGAGCCGATTTTTATGCCGATAATGTCATTCAATTAAAAAAAGAAGGTGAACTAAGTTTGAATA
TCTTTTTAGAACTTTGTAGAGAAAAAGGTATTGAGCCGTTCAAACAATATTCCGGAAAGTTTAATGTTCGCCTTTCGCCT
GAATTACATAAAGCTGCGGTAATTGCAGCAACTGCTGAAAATTTAAGCTTAAATGAATGGATTAACCAAACGTTAGAAAA
GAGCGTGTAG

Structures

Antitoxin

• AlphaFold DB: A0A829CM33