Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 290260..290843 Replicon chromosome
Accession NZ_CP044315
Organism Escherichia coli strain SJ7

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag F7D02_RS01355 Protein ID WP_000254745.1
Coordinates 290508..290843 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag F7D02_RS01350 Protein ID WP_000581937.1
Coordinates 290260..290508 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D02_RS01340 286599..287900 + 1302 WP_000046800.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
F7D02_RS01345 287948..290182 + 2235 WP_000226815.1 GTP pyrophosphokinase -
F7D02_RS01350 290260..290508 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
F7D02_RS01355 290508..290843 + 336 WP_000254745.1 endoribonuclease MazF Toxin
F7D02_RS01360 290914..291705 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
F7D02_RS01365 291933..293570 + 1638 WP_000210883.1 CTP synthase (glutamine hydrolyzing) -
F7D02_RS01370 293658..294956 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T140658 WP_000254745.1 NZ_CP044315:290508-290843 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T140658 NZ_CP059329:1676234-1676503 [Mannheimia haemolytica]
ATGCGAGAGATTGAGTATAGTAATGCTTTTAAACGTGACTATAAAAAATGTGCGGTTGAGTCTTTATCCTCTTCATTAAT
TGAGGCGTTGTATTTTCTGATTAATGACAAGCCATTACCTGAGAAATACTGCGATCACGCTTTAACCGGCGAATGGAAAG
GCTTTAGGGATTGCCATATCCAACCTGATTTGGTTCTTATCTATCGAAAAGTGGAAGATGATAAGCTAGAGCTGCTGCGA
ATTGGCAGCCACTCTCATTTATTTGGCTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT140658 WP_000581937.1 NZ_CP044315:290260-290508 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT140658 NZ_CP059329:1675981-1676247 [Mannheimia haemolytica]
ATGGCAAATAGTGCATTAGTAAGCGTAAGAATCAGCCCTGAAATTAAAGAGCAAGCAAGTGAGGTGTTAGCCGGTATCGG
CTTGACTGTCTCTGATGTGATGCGAATGACATTAGCGAAAATCGCAACAGAAAAACGTTTTCAGTTTGATTATCAGCCTA
ATTTTGAGACAGCAGAAGTATTAAGGGCTGTTAAGAATGGCGAGGAAAAGCTACATTCAGCAAATAGTATTGCGGATTTA
ATGGAGCAACTCAATGCGAGAGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References