Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 120139..120793 Replicon chromosome
Accession NZ_CP044315
Organism Escherichia coli strain SJ7

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag F7D02_RS00615 Protein ID WP_000244772.1
Coordinates 120386..120793 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag F7D02_RS00610 Protein ID WP_000354046.1
Coordinates 120139..120405 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D02_RS00585 115426..116169 + 744 WP_001436959.1 SDR family oxidoreductase -
F7D02_RS00590 116226..117659 - 1434 WP_001342496.1 6-phospho-beta-glucosidase BglA -
F7D02_RS00595 117704..118015 + 312 WP_001182947.1 N(4)-acetylcytidine aminohydrolase -
F7D02_RS00600 118179..118838 + 660 WP_000250274.1 hemolysin III family protein -
F7D02_RS00605 118916..119896 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
F7D02_RS00610 120139..120405 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
F7D02_RS00615 120386..120793 + 408 WP_000244772.1 protein YgfX Toxin
F7D02_RS00620 120833..121354 - 522 WP_001055874.1 flavodoxin FldB -
F7D02_RS00625 121466..122362 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
F7D02_RS00630 122387..123097 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
F7D02_RS00635 123103..124836 + 1734 WP_000813223.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T140657 WP_000244772.1 NZ_CP044315:120386-120793 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T140657 NZ_CP059329:c1632926-1632594 [Mannheimia haemolytica]
ATGAAACGTGGCGATATTTACTGGCTCGATCTTGATCCAACCTTAGGACACGAACAATCAGGCTTCCGCCCCGTTGTAAT
TGTTTCCGCAACTGCATTTAATCTAGCCAGTAAACTGCCTGTGGTTGTGCCGATTACTAACGGTGGAGCTTTTGCTGAAA
GACTGGGCTTTGCCGTTTCATTAAAAGATGCCGGCTTAAAAACAACAGGGATTATTCGATGCGATCAACCTCGCACAGTC
GATATTGCACAACGCCATGGAAAATTTATCGAAGCCTTACCCGAAAGCCTACTTGATGAAATTATGGCAAGGGTTTCAGT
GATTTTTGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT140657 WP_000354046.1 NZ_CP044315:120139-120405 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT140657 NZ_CP059329:c1633183-1632923 [Mannheimia haemolytica]
ATGCCAACAATCGCATTACGTCAGGTCGGTGGCTCGGTTGCAATGGTGATTCCGCCTGCTTTTTTAGAGGCACTCAAGCT
CTCCGTCGGTAGCAAAGTGGATTTGTCTTTAAACGACAACCAACTCGTGATTAATCCTGCCCGCCCAAAAGTGAAATACA
GCTTAGAGGAATTATTAAGACAATCCGATTTTGAGGCAGCAAGGAATGAAGAAAGCCAATCGTGGTTATCCGAGCCGGCA
ATAGGAAAAGAAATTTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References