Detailed information of TA system TA140646

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3959244..3959723
• Replicon: chromosome
• Accession: NZ_CP044314
• Organism: Escherichia coli strain RM9245

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0P7S0Y8
• Locus tag: F7D03_RS19300
• Protein ID: WP_032211256.1
• Coordinates: 3959244..3959531 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: -
• Locus tag: F7D03_RS19305
• Protein ID: WP_064579147.1
• Coordinates: 3959532..3959723 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D03_RS19265	3954385..3954654	-	270	WP_054250470.1	excisionase	-
F7D03_RS19270	3954716..3957187	-	2472	WP_096560541.1	exonuclease	-
F7D03_RS19275	3957283..3957471	-	189	WP_064579146.1	DUF1482 family protein	-
F7D03_RS19280	3957468..3957656	-	189	WP_000450222.1	cell division inhibitor	-
F7D03_RS19290	3958185..3958700	+	516	WP_000559918.1	hypothetical protein	-
F7D03_RS19295	3958814..3958966	-	153	WP_000380318.1	DUF1391 family protein	-
F7D03_RS19300	3959244..3959531	-	288	WP_032211256.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F7D03_RS19305	3959532..3959723	-	192	WP_064579147.1	hypothetical protein	Antitoxin
F7D03_RS19310	3959692..3960153	-	462	WP_064579148.1	helix-turn-helix domain-containing protein	-
F7D03_RS19315	3960173..3960544	-	372	WP_000448216.1	hypothetical protein	-
F7D03_RS19320	3960647..3960928	+	282	WP_021577226.1	helix-turn-helix domain-containing protein	-
F7D03_RS19325	3960932..3961357	+	426	WP_000693827.1	Rha family transcriptional regulator	-
F7D03_RS19330	3961426..3962457	+	1032	WP_052909572.1	phage replisome organizer	-
F7D03_RS19335	3962369..3962911	+	543	WP_072130322.1	replication protein	-
F7D03_RS19340	3962945..3963661	+	717	WP_047082289.1	DUF1627 domain-containing protein	-
F7D03_RS19345	3963694..3963975	+	282	WP_072094476.1	DNA-binding protein	-
F7D03_RS19350	3963972..3964277	+	306	WP_053294093.1	DUF4406 domain-containing protein	-
F7D03_RS19355	3964425..3964607	+	183	WP_022581296.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	stx2A / stx2B	3946129..4103277	157148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11013.72 Da
• Isoelectric Point: 6.2118

>T140646 WP_032211256.1 NZ_CP044314:c3959531-3959244 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWKRLRTCVQPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTISGIE
IVNVVHARRQFPVTP

Nucleotide

• Length: 288 bp

>T140646 NZ_CP059328:c983214-982945 [Mannheimia haemolytica]
ATGCGAGAGATTGAGTATAGTAATGCTTTTAAACGTGACTATAAATAATGTGCGGTTGAGTCTTTATCCTCTTCATTAAT
TGAGGCGTTGTATTTTCTGATTAATGACAAGCCATTACCTGAGAAATACTGCGATCACGCTTTAACCGGCGAATGGAAAG
GTTTTAGGGATTGCCATATCCAACCTGATTTGGTTCTTATCTATCGAAAAGTGGAAGATGATAAGCTAGAGCTGCTGCGA
ATTGGCAGCCACTCTCATTTATTTGGCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7395.30 Da
• Isoelectric Point: 5.1069

>AT140646 WP_064579147.1 NZ_CP044314:c3959723-3959532 [Escherichia coli]
MNTTLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDEVERRMAERFAKMRKERSEQ

Nucleotide

• Length: 192 bp

>AT140646 NZ_CP059328:c983467-983201 [Mannheimia haemolytica]
ATGGCAAATAGTGCATTAGTAAGCGTAAGAATCAGCCCTGAAATTAAAGAGCAAGCAAGTGAGGTGTTAGCCGGTATCGG
CTTGACTGTCTCTGATGTGATGCGAATGACATTAGCGAAAATCGCAACAGAAAAACGTTTTCAGTTTGATTATCAGCCTA
ATTTTGAGACAGCAGAAGTATTAAGGGCTGTTAAGAATGGCGAGGAAAAGCTACATTCAGCAAATAGTATTGCGGATTTA
ATGGAGCAACTCAATGCGAGAGATTGA

Structures

Toxin

• AlphaFold DB: A0A0P7S0Y8