140644

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3166108..3166945 Replicon chromosome
Accession NZ_CP044314
Organism Escherichia coli strain RM9245

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag F7D03_RS15400 Protein ID WP_000227784.1
Coordinates 3166108..3166650 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag F7D03_RS15405 Protein ID WP_001297137.1
Coordinates 3166634..3166945 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D03_RS15380 3161647..3162558 - 912 WP_000705853.1 2-dehydropantoate 2-reductase -
F7D03_RS15385 3162726..3163217 + 492 WP_001138904.1 nucleotide binding protein YajQ -
F7D03_RS15390 3163345..3164709 - 1365 WP_001000978.1 MFS transporter -
F7D03_RS15395 3165117..3166052 + 936 WP_001297127.1 sel1 repeat family protein -
F7D03_RS15400 3166108..3166650 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
F7D03_RS15405 3166634..3166945 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
F7D03_RS15410 3167130..3168020 - 891 WP_000971336.1 heme o synthase -
F7D03_RS15415 3168032..3168361 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
F7D03_RS15420 3168361..3168975 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
F7D03_RS15425 3168965..3170956 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
F7D03_RS15430 3170978..3171925 - 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T140644 WP_000227784.1 NZ_CP044314:c3166650-3166108 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T140644 NZ_CP059327:2455843-2456232 [Mannheimia haemolytica]
ATGTATATGTTAGATACGAATACTGTAAGCTAATTTTTTCGTGGAAATGCAAATGTGGTGGCGAAATTGAAGTCCATTAA
TCCTGAGAGGCTTTGTATTTCATCTGTAACTGCTGCTGAATTGGTTTATGGTGTGGCGAAAAGAAATAATGGGCAGCTTT
CTCAATTTTTAGGTTATTTTTTATCGAGTGTAAAGGTTTTAGATTGGAATTATCGTTGTGCTGAACTTTATGGAAAGTTA
CGGGCAGAGATGGAGAAAAATGGCAAAGTAATGGGCGTGCAGGATCAAATGATTGCTAGTCACGCTCTTGCTGAAGAGTG
TATTTTAGTTTCTAGCGATCAGGCATTTAAAATGGTACCTGATCTGAAATTGGAGAATTGGTTACACTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT140644 WP_001297137.1 NZ_CP044314:c3166945-3166634 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT140644 NZ_CP059327:2455579-2455842 [Mannheimia haemolytica]
ATGGAAAAAATTGCGAGATTATTCCGTAACGGGCGGAACCAAGCAGTGCGTTTACCGGTTGAATTTGAGTTTGATGCAGA
GCAAGTCTATGTTTCTAAACAAGAAAATGGCGATATTTTATTATCATTGAGATCGAGAAATACCGAGCATTGGCAACGAT
TGTTTGAGCTATTGCCTGCTGTTGTATGTGATGAAACATTTTTAGATACAAAAAAAGAGAGAAACCAATCCTTTGAGCAA
CGGGATCCTTTTGAGGTAGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References