Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 1574628..1575428 Replicon chromosome
Accession NZ_CP044314
Organism Escherichia coli strain RM9245

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag F7D03_RS07810 Protein ID WP_000342449.1
Coordinates 1574628..1575155 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag F7D03_RS07815 Protein ID WP_001277108.1
Coordinates 1575162..1575428 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D03_RS07785 1569703..1570470 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
F7D03_RS07790 1570467..1571744 - 1278 WP_000803771.1 branched chain amino acid ABC transporter permease LivM -
F7D03_RS07795 1571741..1572667 - 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
F7D03_RS07800 1572715..1573824 - 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
F7D03_RS07805 1574248..1574631 + 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
F7D03_RS07810 1574628..1575155 - 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
F7D03_RS07815 1575162..1575428 - 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
F7D03_RS07820 1575578..1576681 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
F7D03_RS07825 1576952..1577806 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
F7D03_RS07830 1578051..1579109 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
F7D03_RS07835 1579102..1579770 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T140639 WP_000342449.1 NZ_CP044314:c1575155-1574628 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T140639 NZ_CP059327:1333073-1333255 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT140639 WP_001277108.1 NZ_CP044314:c1575428-1575162 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT140639 NZ_CP059327:1333294-1333713 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATACCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGTAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACGCT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References