Detailed information of TA system TA140637

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1073905..1074740
• Replicon: chromosome
• Accession: NZ_CP044314
• Organism: Escherichia coli strain RM9245

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: F7D03_RS05290
• Protein ID: WP_000854821.1
• Coordinates: 1074363..1074740 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: F7D03_RS05285
• Protein ID: WP_001285610.1
• Coordinates: 1073905..1074273 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D03_RS05250	1070799..1071254	+	456	WP_000581504.1	hypothetical protein	-
F7D03_RS05255	1071333..1071566	+	234	WP_001119729.1	DUF905 family protein	-
F7D03_RS05265	1071666..1072485	+	820	Protein_996	DUF945 domain-containing protein	-
F7D03_RS05270	1072540..1073025	+	486	WP_000849580.1	antirestriction protein	-
F7D03_RS05275	1073041..1073517	+	477	WP_001186727.1	RadC family protein	-
F7D03_RS05280	1073604..1073825	+	222	WP_000692350.1	DUF987 domain-containing protein	-
F7D03_RS05285	1073905..1074273	+	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
F7D03_RS05290	1074363..1074740	+	378	WP_000854821.1	type IV toxin-antitoxin system toxin CbtA	Toxin
F7D03_RS05295	1074737..1074934	+	198	Protein_1002	hypothetical protein	-
F7D03_RS05300	1074962..1075159	+	198	WP_085949090.1	DUF957 domain-containing protein	-
F7D03_RS05305	1075244..1076086	+	843	WP_001280493.1	DUF4942 domain-containing protein	-
F7D03_RS05310	1076367..1076903	-	537	WP_000942794.1	GspM family type II secretion system protein YghD	-
F7D03_RS05315	1076905..1078083	-	1179	WP_000094989.1	type II secretion system protein GspL	-
F7D03_RS05320	1078080..1079057	-	978	WP_000633239.1	type II secretion system minor pseudopilin GspK	-
F7D03_RS05325	1079054..1079659	-	606	WP_001255030.1	type II secretion system minor pseudopilin GspJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	gspM / gspL / gspK / gspJ / gspI / gspH / gspG / gspF / gspE / gspD / gspC	997260..1135933	138673

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T140637 WP_000854821.1 NZ_CP044314:1074363-1074740 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T140637 NZ_CP059326:1806478-1806774 [Mannheimia haemolytica]
ATGTATATAGATTTGCAATATGCTGTGCCGTTATTGTTTGAATACGACCCGAACAAAAGCCAAATTAATTTAGCCAAATA
TGGTATTGATTTTGAACAGGCAAAGTTGCTTTGGGAAGATGAACGAAAAGTTGTACTGGAGGCTTGCATTGAACCCGAAT
TACGCTATTTCCTGATTGGCAAGATTTATGATAAATATTGGACAGCGTTTTTTACACCAAGAAATGATGTTATTCGTTTA
ATTTCAGTTAGACGTTCCCGTAAAAAAGAGATCTCATACTATGAGAAATACCATTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT140637 WP_001285610.1 NZ_CP044314:1073905-1074273 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT140637 NZ_CP059326:1806758-1807000 [Mannheimia haemolytica]
ATGAGAAATACCATTAGTGCCGAAGAATTAGAACGTATCTTCGATGAAGGCAAAGAAGATATTTTACAATACTGCGATCT
TTCTACCGCTCATCGCCCGGGGCAAATGAAAAGGGTGAATGTTGATTTCCCAAATTGGATGGTAGAAGCGTTAGATAAGC
AAGCCAGACATTTAGGTGTGCCTCGCCAAGCCTTGAGTAAAATGTGGATTGCGGAAAAATTACCGGTTCAATCACCTAGG
TAA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54