Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 913453..914107 Replicon chromosome
Accession NZ_CP044314
Organism Escherichia coli strain RM9245

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag F7D03_RS04475 Protein ID WP_000244772.1
Coordinates 913453..913860 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag F7D03_RS04480 Protein ID WP_000354046.1
Coordinates 913841..914107 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D03_RS04455 909410..911143 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
F7D03_RS04460 911149..911859 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
F7D03_RS04465 911884..912780 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
F7D03_RS04470 912892..913413 + 522 WP_001055874.1 flavodoxin FldB -
F7D03_RS04475 913453..913860 - 408 WP_000244772.1 protein YgfX Toxin
F7D03_RS04480 913841..914107 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
F7D03_RS04485 914350..915330 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
F7D03_RS04490 915407..916066 - 660 WP_000250274.1 hemolysin III family protein -
F7D03_RS04495 916230..916541 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
F7D03_RS04500 916586..918019 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
F7D03_RS04505 918076..918819 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T140636 WP_000244772.1 NZ_CP044314:c913860-913453 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T140636 NZ_CP059326:1578255-1578530 [Mannheimia haemolytica]
ATGGGTATCACCGAATTTGATGCCGCCGAATACCTCACCAATGACGAGCTAATCGCCCTTTATTTGGCGGAAACATTGAA
AGACGGCACGGACGAGGAGTTTATTCAGGCATTGAATACCGTGGCACGAGCGAAAGGAATGAATGAGTTAGCGAAAAAAG
CAGGGATCGGGCGTGAAAGTTTGTATCAAAGCCTTTCCAGCAGTAAGCCCCGTTTTGAGACTATCCGCAAAATTATTAGC
GCATTAAATTTGGAGTTGTCGGTCGTTAAGGCGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT140636 WP_000354046.1 NZ_CP044314:c914107-913841 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT140636 NZ_CP059326:1577888-1578139 [Mannheimia haemolytica]
ATGTACCATATTGAACGCACTGAAATTTTCGACCAATGGCTTGAAAATTTAAAAGATCCACTTGCTGTGGTGACCATTGC
AAGACGTATTGAACGTGCCGAAAGCGGAAATTTTGGCGATACCAAATATATCGCAGAAGGAATTTCCGAAATGAGATTAA
ACGTCAGCAAAGGTTATCGCCTTTACTATGCACAAAAAGGTAGCATTATTTATATACTACTAAATGGAGGATATAAAGGC
ACGCCAAGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References