Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 777190..777773 Replicon chromosome
Accession NZ_CP044314
Organism Escherichia coli strain RM9245

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag F7D03_RS03830 Protein ID WP_000254745.1
Coordinates 777190..777525 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag F7D03_RS03835 Protein ID WP_000581937.1
Coordinates 777525..777773 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D03_RS03810 772659..773021 + 363 WP_000034929.1 hypothetical protein -
F7D03_RS03815 773077..774375 - 1299 WP_000036723.1 phosphopyruvate hydratase -
F7D03_RS03820 774463..776100 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
F7D03_RS03825 776328..777119 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
F7D03_RS03830 777190..777525 - 336 WP_000254745.1 endoribonuclease MazF Toxin
F7D03_RS03835 777525..777773 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
F7D03_RS03840 777851..780085 - 2235 WP_000226815.1 GTP pyrophosphokinase -
F7D03_RS03845 780133..781434 - 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T140635 WP_000254745.1 NZ_CP044314:c777525-777190 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T140635 NZ_CP059326:1577888-1578139 [Mannheimia haemolytica]
ATGTACCATATTGAACGCACTGAAATTTTCGACCAATGGCTTGAAAATTTAAAAGATCCACTTGCTGTGGTGACCATTGC
AAGACGTATTGAACGTGCCGAAAGCGGAAATTTTGGCGATACCAAATATATCGCAGAAGGAATTTCCGAAATGAGATTAA
ACGTCAGCAAAGGTTATCGCCTTTACTATGCACAAAAAGGTAGCATTATTTATATACTACTAAATGGAGGATATAAAGGC
ACGCCAAGCTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT140635 WP_000581937.1 NZ_CP044314:c777773-777525 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT140635 NZ_CP059326:1578255-1578530 [Mannheimia haemolytica]
ATGGGTATCACCGAATTTGATGCCGCCGAATACCTCACCAATGACGAGCTAATCGCCCTTTATTTGGCGGAAACATTGAA
AGACGGCACGGACGAGGAGTTTATTCAGGCATTGAATACCGTGGCACGAGCGAAAGGAATGAATGAGTTAGCGAAAAAAG
CAGGGATCGGGCGTGAAAGTTTGTATCAAAGCCTTTCCAGCAGTAAGCCCCGTTTTGAGACTATCCGCAAAATTATTAGC
GCATTAAATTTGGAGTTGTCGGTCGTTAAGGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References