Detailed information of TA system TA140632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4664699..4665536
• Replicon: chromosome
• Accession: NZ_CP044313
• Organism: Escherichia coli strain RM11911

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: F7D04_RS22985
• Protein ID: WP_000227784.1
• Coordinates: 4664699..4665241 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: -
• Locus tag: F7D04_RS22990
• Protein ID: WP_050938765.1
• Coordinates: 4665225..4665536 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D04_RS22965	4660238..4661149	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
F7D04_RS22970	4661317..4661808	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
F7D04_RS22975	4661936..4663300	-	1365	WP_001000978.1	MFS transporter	-
F7D04_RS22980	4663708..4664643	+	936	WP_001297127.1	sel1 repeat family protein	-
F7D04_RS22985	4664699..4665241	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
F7D04_RS22990	4665225..4665536	-	312	WP_050938765.1	DUF1778 domain-containing protein	Antitoxin
F7D04_RS22995	4665721..4666611	-	891	WP_000971336.1	heme o synthase	-
F7D04_RS23000	4666623..4666952	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
F7D04_RS23005	4666952..4667566	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
F7D04_RS23010	4667556..4669547	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
F7D04_RS23015	4669569..4670516	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T140632 WP_000227784.1 NZ_CP044313:c4665241-4664699 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T140632 NZ_CP059326:753423-753755 [Mannheimia haemolytica]
ATGAAACGTGGCGATATTTACTGGCTCGATCTTGATCCAACCTTAGGACACGAACAATCAGGCTTCCGCCCCGTTGTAAT
TGTTTCCGCAACTGCATTTAATCTAGCCAGTAAACTGCCTGTGGTTGTGCCGATTACTAACGGTGGAGCTTTTGCTGAAA
GACTGGGCTTTGCCGTTTCATTAAAAGATGCCGGCTTAAAAACAACAGGGATTATTCGATGCGATCAACCTCGCACAGTC
GATATTGCACAACGCCATGGAAAATTTATCGAAGCCTTACCCGAAAGCCTACTTGATGAAATTATGGCAAGGGTTTCAGT
GATTTTTGAGTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11357.05 Da
• Isoelectric Point: 10.6798

>AT140632 WP_050938765.1 NZ_CP044313:c4665536-4665225 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLNNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT140632 NZ_CP059326:753166-753426 [Mannheimia haemolytica]
ATGCCAACAATCGCATTACGTCAGGTCGGTGGCTCGGTTGCAATGGTGATTCCGCCTGCTTTTTTAGAGGCACTCAAGCT
CTCCGTCGGTAGCAAAGTGGATTTGTCTTTAAACGACAACCAACTCGTGATTAATCCTGCCCGCCCAAAAGTGAAATACA
GCTTAGAGGAATTATTAAGACAATCCGATTTTGAGGCAGCAAGGAATGAAGAAAGCCAATCGTGGTTATCCGAGCCGGCA
ATAGGAAAAGAAATTTTATGA

Structures

Toxin

• PDB: 7BYY