Detailed information of TA system TA140621

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1859216..1859841
• Replicon: chromosome
• Accession: NZ_CP044313
• Organism: Escherichia coli strain RM11911

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: F7D04_RS09415
• Protein ID: WP_000911330.1
• Coordinates: 1859216..1859614 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: F7D04_RS09420
• Protein ID: WP_000450524.1
• Coordinates: 1859614..1859841 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D04_RS09395	1855094..1855294	+	201	WP_000383836.1	YpfN family protein	-
F7D04_RS09400	1855404..1856102	-	699	WP_000679823.1	esterase	-
F7D04_RS09405	1856176..1858191	-	2016	WP_000829294.1	tRNA cytosine(34) acetyltransferase TmcA	-
F7D04_RS09410	1858206..1859069	-	864	WP_050938869.1	neutral zinc metallopeptidase	-
F7D04_RS09415	1859216..1859614	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
F7D04_RS09420	1859614..1859841	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
F7D04_RS09425	1859994..1860707	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
F7D04_RS09430	1860920..1861954	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
F7D04_RS09435	1861971..1862849	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
F7D04_RS09440	1862995..1863567	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
F7D04_RS09445	1863567..1864037	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T140621 WP_000911330.1 NZ_CP044313:c1859614-1859216 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T140621 NZ_CP059325:565988-566320 [Mannheimia haemolytica]
ATGAAACGTGGCGATATTTACTGGCTCGATCTTGATCCAACCTTAGGACACGAACAATCAGGCTTCCGCCCCGTTGTAAT
TGTTTCCGCAACTGCATTTAATCTAGCCAGTAAACTGCCTGTGGTTGTGCCGATTACTAACGGTGGAGCTTTTGCTGAAA
GACTGGGCTTTGCCGTTTCATTAAAAGATGCCGGCTTAAAAACAACAGGGATTATTCGATGCGATCAACCTCGCACAGTC
GATATTGCACAACGCCATGGAAAATTTATCGAAGCCTTACCCGAAAGCCTACTTGATGAAATTATGGCAAGGGTTTCAGT
GATTTTTGAGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT140621 WP_000450524.1 NZ_CP044313:c1859841-1859614 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT140621 NZ_CP059325:565731-565991 [Mannheimia haemolytica]
ATGCCAACAATCGCATTACGTCAGGTCGGTGGCTCGGTTGCAATGGTGATTCCGCCTGCTTTTTTAGAGGCACTCAAGCT
CTCCGTCGGTAGCAAAGTGGATTTGTCTTTAAACGACAACCAACTCGTGATTAATCCTGCCCGCCCAAAAGTGAAATACA
GCTTAGAGGAATTATTAAGACAATCCGATTTTGAGGCAGCAAGGAATGAAGAAAGCCAATCGTGGTTATCCGAGCCGGCA
ATAGGAAAAGAAATTTTATGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33