Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 594919..595557 Replicon chromosome
Accession NZ_CP044313
Organism Escherichia coli strain RM11911

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag F7D04_RS03190 Protein ID WP_000813794.1
Coordinates 594919..595095 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag F7D04_RS03195 Protein ID WP_001270286.1
Coordinates 595141..595557 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D04_RS03170 590532..591746 - 1215 WP_001323465.1 BenE family transporter YdcO -
F7D04_RS03175 591799..592335 + 537 WP_000429152.1 DNA-binding transcriptional regulator SutR -
F7D04_RS03180 592408..594375 + 1968 WP_050938771.1 23S rRNA 5-hydroxycytidine C2501 synthase -
F7D04_RS03185 594467..594697 - 231 WP_000494244.1 YncJ family protein -
F7D04_RS03190 594919..595095 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
F7D04_RS03195 595141..595557 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
F7D04_RS03200 595636..597042 + 1407 WP_000760629.1 PLP-dependent aminotransferase family protein -
F7D04_RS03205 597287..598432 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
F7D04_RS03210 598450..599463 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
F7D04_RS03215 599464..600405 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 589344..590492 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T140610 WP_000813794.1 NZ_CP044313:594919-595095 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T140610 NZ_CP059323:c2427101-2426769 [Mannheimia haemolytica]
ATGAAACGTGGCGATATTTACTGGCTCGATCTTGATCCAACCTTAGGACACGAACAATCAGGCTTCCGCCCCGTTGTAAT
TGTTTCCGCAACTGCATTTAATCTAGCCAGTAAACTGCCTGTGGTTGTGCCGATTACTAACGGTGGAGCTTTTGCTGAAA
GACTGGGCTTTGCCGTTTCATTAAAAGATGCCGGCTTAAAAACAACAGGGATTATTCGATGCGATCAACCTCGCACAGTC
GATATTGCACAACGCCATGGAAAATTTATCGAAGCCTTACCCGAAAGCCTACTTGATGAAATTATGGCAAGGGTTTCAGT
GATTTTTGAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT140610 WP_001270286.1 NZ_CP044313:595141-595557 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT140610 NZ_CP059323:c2427358-2427098 [Mannheimia haemolytica]
ATGCCAACAATCGCATTACGTCAGGTCGGTGGCTCGGTTGCAATGGTGATTCCGCCTGCTTTTTTAGAGGCACTCAAGCT
CTCCGTCGGTAGCAAAGTGGATTTGTCTTTAAACGACAACCAACTCGTGATTAATCCTGCCCGCCCAAAAGTGAAATACA
GCTTAGAGGAATTATTAAGACAATCCGATTTTGAGGCAGCAAGGAATGAAGAAAGCCAATCGTGGTTATCCGAGCCGGCA
ATAGGAAAAGAAATTTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References