Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4608292..4609092 Replicon chromosome
Accession NZ_CP044312
Organism Escherichia coli strain RM13745

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag F7D05_RS22745 Protein ID WP_000342449.1
Coordinates 4608292..4608819 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag F7D05_RS22750 Protein ID WP_001277108.1
Coordinates 4608826..4609092 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D05_RS22720 4603367..4604134 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
F7D05_RS22725 4604131..4605408 - 1278 WP_050939128.1 branched chain amino acid ABC transporter permease LivM -
F7D05_RS22730 4605405..4606331 - 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
F7D05_RS22735 4606379..4607488 - 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
F7D05_RS22740 4607912..4608295 + 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
F7D05_RS22745 4608292..4608819 - 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
F7D05_RS22750 4608826..4609092 - 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
F7D05_RS22755 4609242..4610345 - 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
F7D05_RS22760 4610617..4611471 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
F7D05_RS22765 4611716..4612774 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
F7D05_RS22770 4612767..4613435 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T140603 WP_000342449.1 NZ_CP044312:c4608819-4608292 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T140603 NZ_CP059319:c3230636-3230448 [Rhizorhabdus wittichii]
ATGCGAAGCGCTGACGTCATCAAGAAGTTGGAGGCTGATGGTTGGAGAGAGGTACGGCAGAGCGGAAGTCACAAGCAGTT
CCGCCACCCGACGAAGCCCGGCACCGTCACCGTGCCCCACCCGAAGAAGGAGATGACCACAGGGACGTTGAAAAGCATCG
AAAAGCAGAGCGGAGTGGGGCTTCGGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT140603 WP_001277108.1 NZ_CP044312:c4609092-4608826 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT140603 NZ_CP059319:c3230416-3230027 [Rhizorhabdus wittichii]
ATGACCACGATCTTCTATCCGGCCATCATCGAACGAGCGCCCGATGGCTATGGCGTGTTCTTTCCCGACCTGCCTGGATG
CACCTCGGGCGGCCGCACCGTGCAGGAAGCCGCTCGCATGGCCGAGGAAGCGCTGGACGGGCATCTTCTCGTCACGGCCC
AGCACGGCGAAACCTTGCCCGAGCCGTCCGAGCTTGATGCGGTCGAGCGTGATCCCGAGGTCGACGAGGTCGCCCGCATC
CTGGTCCGCGCGCAGAAACCCGGCCGGGCCATTCGCATCAATATCACCATGGATGAGGGCCTGGTCGCACGGATCGACCG
CGCATCGAAGAACCGTTCGCGGTTCATCGCCGATGCGGCGCGCATGGCGCTCGCGTCGACCGAAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References