Detailed information of TA system TA140597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3442830..3443455
• Replicon: chromosome
• Accession: NZ_CP044312
• Organism: Escherichia coli strain RM13745

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: F7D05_RS16920
• Protein ID: WP_000911330.1
• Coordinates: 3442830..3443228 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: F7D05_RS16925
• Protein ID: WP_000450524.1
• Coordinates: 3443228..3443455 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D05_RS16900	3438708..3438908	+	201	WP_000383836.1	YpfN family protein	-
F7D05_RS16905	3439018..3439716	-	699	WP_000679823.1	esterase	-
F7D05_RS16910	3439790..3441805	-	2016	WP_000829294.1	tRNA cytosine(34) acetyltransferase TmcA	-
F7D05_RS16915	3441820..3442683	-	864	WP_050938869.1	neutral zinc metallopeptidase	-
F7D05_RS16920	3442830..3443228	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
F7D05_RS16925	3443228..3443455	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
F7D05_RS16930	3443608..3444321	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
F7D05_RS16935	3444534..3445568	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
F7D05_RS16940	3445585..3446463	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
F7D05_RS16945	3446609..3447181	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
F7D05_RS16950	3447181..3447651	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T140597 WP_000911330.1 NZ_CP044312:c3443228-3442830 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T140597 NZ_CP059317:c5257698-5257315 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT140597 WP_000450524.1 NZ_CP044312:c3443455-3443228 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT140597 NZ_CP059317:c5257940-5257698 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33