Detailed information of TA system TA140595

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2858777..2859608
• Replicon: chromosome
• Accession: NZ_CP044312
• Organism: Escherichia coli strain RM13745

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: F7D05_RS14300
• Protein ID: WP_000854814.1
• Coordinates: 2859234..2859608 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0H0EA76
• Locus tag: F7D05_RS14295
• Protein ID: WP_001285592.1
• Coordinates: 2858777..2859145 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D05_RS14265	2855681..2856136	+	456	WP_000581504.1	hypothetical protein	-
F7D05_RS14270	2856215..2856448	+	234	WP_001119724.1	DUF905 family protein	-
F7D05_RS14275	2856548..2857366	+	819	WP_047090156.1	DUF945 domain-containing protein	-
F7D05_RS14280	2857448..2857927	+	480	WP_000860086.1	antirestriction protein	-
F7D05_RS14285	2857943..2858419	+	477	WP_001186726.1	RadC family protein	-
F7D05_RS14290	2858482..2858703	+	222	WP_000692323.1	DUF987 domain-containing protein	-
F7D05_RS14295	2858777..2859145	+	369	WP_001285592.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
F7D05_RS14300	2859234..2859608	+	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
F7D05_RS14305	2859605..2859799	+	195	WP_000988600.1	hypothetical protein	-
F7D05_RS14310	2859812..2859925	+	114	WP_001161660.1	DUF957 domain-containing protein	-
F7D05_RS14315	2860214..2860342	-	129	Protein_2801	transposase domain-containing protein	-
F7D05_RS14320	2860414..2860596	+	183	WP_001016348.1	hypothetical protein	-
F7D05_RS14325	2860697..2861026	-	330	WP_000450409.1	DUF496 family protein	-
F7D05_RS14330	2861198..2862256	-	1059	WP_001200891.1	FUSC family protein	-
F7D05_RS14335	2862454..2862927	-	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
F7D05_RS14340	2863046..2864212	-	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T140595 WP_000854814.1 NZ_CP044312:2859234-2859608 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T140595 NZ_CP059317:c4773678-4773394 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13636.51 Da
• Isoelectric Point: 6.6249

>AT140595 WP_001285592.1 NZ_CP044312:2858777-2859145 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT140595 NZ_CP059317:c4773910-4773668 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A0H0EA76