Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2179796..2180434 Replicon chromosome
Accession NZ_CP044312
Organism Escherichia coli strain RM13745

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag F7D05_RS10710 Protein ID WP_000813794.1
Coordinates 2179796..2179972 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag F7D05_RS10715 Protein ID WP_001270286.1
Coordinates 2180018..2180434 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D05_RS10690 2175409..2176623 - 1215 WP_001323465.1 BenE family transporter YdcO -
F7D05_RS10695 2176676..2177212 + 537 WP_000429152.1 DNA-binding transcriptional regulator SutR -
F7D05_RS10700 2177285..2179252 + 1968 WP_050938771.1 23S rRNA 5-hydroxycytidine C2501 synthase -
F7D05_RS10705 2179344..2179574 - 231 WP_000494244.1 YncJ family protein -
F7D05_RS10710 2179796..2179972 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
F7D05_RS10715 2180018..2180434 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
F7D05_RS10720 2180513..2181919 + 1407 WP_000760629.1 PLP-dependent aminotransferase family protein -
F7D05_RS10725 2182164..2183309 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
F7D05_RS10730 2183327..2184340 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
F7D05_RS10735 2184341..2185282 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2174221..2175369 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T140586 WP_000813794.1 NZ_CP044312:2179796-2179972 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T140586 NZ_CP059317:331123-331491 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGACAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT140586 WP_001270286.1 NZ_CP044312:2180018-2180434 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT140586 NZ_CP059317:330905-331126 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References