Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1018178..1018796 Replicon chromosome
Accession NZ_CP044312
Organism Escherichia coli strain RM13745

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag F7D05_RS04865 Protein ID WP_001291435.1
Coordinates 1018178..1018396 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag F7D05_RS04870 Protein ID WP_000344800.1
Coordinates 1018422..1018796 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D05_RS04830 1013467..1014039 + 573 WP_000779831.1 YbaY family lipoprotein -
F7D05_RS04835 1014070..1014381 - 312 WP_000409911.1 MGMT family protein -
F7D05_RS04845 1014760..1015113 + 354 WP_000878140.1 DUF1428 domain-containing protein -
F7D05_RS04850 1015155..1016705 - 1551 WP_001514985.1 cyclic-guanylate-specific phosphodiesterase PdeB -
F7D05_RS04855 1016869..1017339 - 471 WP_000136192.1 YlaC family protein -
F7D05_RS04860 1017455..1018006 - 552 WP_001727569.1 maltose O-acetyltransferase -
F7D05_RS04865 1018178..1018396 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
F7D05_RS04870 1018422..1018796 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
F7D05_RS04875 1019342..1022491 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
F7D05_RS04880 1022514..1023707 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T140581 WP_001291435.1 NZ_CP044312:c1018396-1018178 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T140581 NZ_CP059312:c4729170-4728886 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT140581 WP_000344800.1 NZ_CP044312:c1018796-1018422 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT140581 NZ_CP059312:c4729402-4729160 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References