Detailed information of TA system TA140580

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 983882..984719
• Replicon: chromosome
• Accession: NZ_CP044312
• Organism: Escherichia coli strain RM13745

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: F7D05_RS04695
• Protein ID: WP_000227784.1
• Coordinates: 983882..984424 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: -
• Locus tag: F7D05_RS04700
• Protein ID: WP_050938765.1
• Coordinates: 984408..984719 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D05_RS04675	979421..980332	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
F7D05_RS04680	980500..980991	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
F7D05_RS04685	981119..982483	-	1365	WP_001000978.1	MFS transporter	-
F7D05_RS04690	982891..983826	+	936	WP_001297127.1	sel1 repeat family protein	-
F7D05_RS04695	983882..984424	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
F7D05_RS04700	984408..984719	-	312	WP_050938765.1	DUF1778 domain-containing protein	Antitoxin
F7D05_RS04705	984904..985794	-	891	WP_000971336.1	heme o synthase	-
F7D05_RS04710	985806..986135	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
F7D05_RS04715	986135..986749	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
F7D05_RS04720	986739..988730	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
F7D05_RS04725	988752..989699	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T140580 WP_000227784.1 NZ_CP044312:c984424-983882 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T140580 NZ_CP044312:c984424-983882 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11357.05 Da
• Isoelectric Point: 10.6798

>AT140580 WP_050938765.1 NZ_CP044312:c984719-984408 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLNNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT140580 NZ_CP044312:c984719-984408 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTAATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY