Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 145494..145991 Replicon chromosome
Accession NZ_CP044312
Organism Escherichia coli strain RM13745

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag F7D05_RS00645 Protein ID WP_001173343.1
Coordinates 145818..145991 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag F7D05_RS00640 Protein ID WP_000398619.1
Coordinates 145494..145790 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D05_RS00620 142225..142944 - 720 WP_000611288.1 two-component system response regulator DcuR -
F7D05_RS00625 142941..144572 - 1632 WP_050939265.1 sensor histidine kinase -
F7D05_RS00630 144753..144983 + 231 WP_000371700.1 (4Fe-4S)-binding protein -
F7D05_RS00635 144995..145267 + 273 WP_000405647.1 N-acetyltransferase -
F7D05_RS00640 145494..145790 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
F7D05_RS00645 145818..145991 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
F7D05_RS00650 146110..147627 - 1518 WP_001295092.1 lysine--tRNA ligase -
F7D05_RS00655 147864..149321 - 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T140577 WP_001173343.1 NZ_CP044312:145818-145991 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T140577 NZ_CP059312:1953991-1954093 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT140577 WP_000398619.1 NZ_CP044312:145494-145790 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT140577 NZ_CP059312:c1954100-1953955 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References