Detailed information of TA system TA140576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4877425..4879108
• Replicon: chromosome
• Accession: NZ_CP044311
• Organism: Escherichia coli strain RM13752

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: I2UPR9
• Locus tag: F7D06_RS23990
• Protein ID: WP_000361917.1
• Coordinates: 4877425..4878342 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: I2UPX5
• Locus tag: F7D06_RS23995
• Protein ID: WP_000157137.1
• Coordinates: 4878335..4879108 (-)
• Length: 258 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D06_RS23970	4872443..4874776	+	2334	WP_136829793.1	toprim domain-containing protein	-
F7D06_RS23975	4875123..4875317	+	195	WP_044815386.1	hypothetical protein	-
F7D06_RS23980	4875535..4876245	+	711	WP_001219054.1	integrase arm-type DNA-binding domain-containing protein	-
F7D06_RS23985	4876249..4876911	+	663	WP_000357928.1	hypothetical protein	-
F7D06_RS23990	4877425..4878342	-	918	WP_000361917.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
F7D06_RS23995	4878335..4879108	-	774	WP_000157137.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
F7D06_RS24000	4879192..4879512	+	321	Protein_4679	IS3 family transposase	-
F7D06_RS24005	4879688..4881037	-	1350	WP_000766442.1	phospholipase	-
F7D06_RS24010	4881156..4883240	-	2085	WP_050939399.1	protease Lon-related BREX system protein BrxL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (13-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34963.04 Da
• Isoelectric Point: 4.7716

>T140576 WP_000361917.1 NZ_CP044311:c4878342-4877425 [Escherichia coli]
MDKTSPYYRQVALLVRALPWVANESCFALKGGTAINLFIHDFPRLSVDIDLAWIPLESREVALPNVREALTRIAAELEAQ
TGIRAVLQTHNPDEMRIIVTTDDAQIKIEVSPVARGTLYPPQERDIVGAVEEEFGFAALPVVSLPDLYGGKLCAALDRQH
PRDFYDVKMLLDTQGIDRSIFNGFIVYLLSHNRPLSEVLNPRWKDITEPFQNEFSGMTFEEVSLEELKEVPERMLSTLKT
HFTQQDFDFLMSFKRGEPDWNLAPEPHIQNLPAVQWKLRNIGQMSEEKRAQSLEKLEKVMTEWLR

Nucleotide

• Length: 918 bp

>T140576 NZ_CP059312:1470321-1470803 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 258 a.a.
• Molecular weight: 29333.76 Da
• Isoelectric Point: 9.9889

>AT140576 WP_000157137.1 NZ_CP044311:c4879108-4878335 [Escherichia coli]
MASKLNWLLQNTSPGSLVLQSWLTRNNISPSLAFKYAQNGWLIKLRAGVYARAGREPDWSDALWCLQNQLEAPVHLAGLS
SLSWQGRSHYLQLKQNQCWLSVENKAILPKWFKEFPAVEWLMVSALKLPDLDEKYRTELEVKGKRITASTPELAAYELLS
AVPQEISFEHAAELFQGLVNLNPRKVEKLLTLSQSVQTKRLYLFFASFYEHAWFKRIDTSKINLGSGNRQIVVNGKLNTQ
YQITVPENFTKKGISHG

Nucleotide

• Length: 774 bp

>AT140576 NZ_CP059312:1470067-1470333 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A0H0PS08

Antitoxin

• AlphaFold DB: A0A0H0N3W2