Detailed information of TA system TA140568

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2964748..2965373
• Replicon: chromosome
• Accession: NZ_CP044311
• Organism: Escherichia coli strain RM13752

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: F7D06_RS14655
• Protein ID: WP_000911330.1
• Coordinates: 2964975..2965373 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: F7D06_RS14650
• Protein ID: WP_000450524.1
• Coordinates: 2964748..2964975 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D06_RS14625	2960552..2961022	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
F7D06_RS14630	2961022..2961594	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
F7D06_RS14635	2961740..2962618	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
F7D06_RS14640	2962635..2963669	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
F7D06_RS14645	2963882..2964595	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
F7D06_RS14650	2964748..2964975	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
F7D06_RS14655	2964975..2965373	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
F7D06_RS14660	2965520..2966383	+	864	WP_050938869.1	neutral zinc metallopeptidase	-
F7D06_RS14665	2966398..2968413	+	2016	WP_000829294.1	tRNA cytosine(34) acetyltransferase TmcA	-
F7D06_RS14670	2968487..2969185	+	699	WP_000679823.1	esterase	-
F7D06_RS14675	2969295..2969495	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T140568 WP_000911330.1 NZ_CP044311:2964975-2965373 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T140568 NZ_CP059308:4039-4314 [Klebsiella pneumoniae]
TTGTCGAAGTGGAATATTAAGTTCACTAGGAATGGCGCTGATGACCTTAACTACTGGCGTGATACTGACAAAAAGAAGTT
TGAGCGTATCCGGCAACTGTTGCTCAGCATCGAGGCTGATCCGCTGACAGGTATAGGAAAACCCGAACGGCTGCGGCACC
ACAAAGATCCGGCACTCTACTCCCGCAGGATTGATCAGAGCCACCGTCTGGTCTATTCAATCCAGAACGGAGAAATCATC
ATCTACGCAGCCCGTTATCACTATGGTGACAAATAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT140568 WP_000450524.1 NZ_CP044311:2964748-2964975 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT140568 NZ_CP059308:3813-4058 [Klebsiella pneumoniae]
ATGCATGCAATCAATTTTACTGATGCCCGTAAACATTTTGCCGAGACGATGAAGCGCGTCACTGATGATGCTGAACCGGT
ACGTGTCATGCGCCGTGATGCGCCTGATATTGTTATGATCGATGCGGGAGAGTATGAGGCGCTAATCGAAACGGTCTATC
TGTTCAGCAATCCGGCGAACGCAGAGCATATCAATGAGTCAATGAAACAGGCGGAGCGCGGCGAATTTGTCGAAGTGGAA
TATTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33